Yang Guang, Rothrauff Benjamin B, Lin Hang, Yu Shuting, Tuan Rocky S
1 Center for Cellular and Molecular Engineering, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania.
2 McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania.
Tissue Eng Part A. 2017 Feb;23(3-4):166-176. doi: 10.1089/ten.TEA.2015.0498. Epub 2017 Jan 10.
Because of the limited and unsatisfactory outcomes of clinical tendon repair, tissue engineering approaches using adult mesenchymal stem cells are being considered a promising alternative strategy to heal tendon injuries. Successful and functional tendon tissue engineering depends on harnessing the biochemical cues presented by the native tendon extracellular matrix (ECM) and the embedded tissue-specific biofactors. In this study, we have prepared and characterized the biological activities of a soluble extract of decellularized tendon ECM (tECM) on adult adipose-derived stem cells (ASCs), on the basis of histological, biochemical, and gene expression analyses. The results showed that tECM enhances the proliferation and transforming growth factor (TGF)-β3-induced tenogenesis of ASCs in both plate and scaffold cultures in vitro, and modulates matrix deposition of ASCs seeded in scaffolds. These findings suggest that combining tendon ECM extract with TGF-β3 treatment is a possible alternative approach to induce tenogenesis for ASCs.
由于临床肌腱修复的效果有限且不尽人意，利用成人间充质干细胞的组织工程方法正被视为一种有前景的替代策略来治疗肌腱损伤。成功且具有功能的肌腱组织工程取决于利用天然肌腱细胞外基质（ECM）呈现的生化线索以及嵌入的组织特异性生物因子。在本研究中，我们基于组织学、生化和基因表达分析，制备并表征了脱细胞肌腱ECM（tECM）的可溶性提取物对成人脂肪来源干细胞（ASC）的生物活性。结果表明，tECM在体外平板和支架培养中均可增强ASC的增殖以及转化生长因子（TGF）-β3诱导的成腱作用，并调节接种于支架中的ASC的基质沉积。这些发现表明，将肌腱ECM提取物与TGF-β3处理相结合是诱导ASC成腱的一种可能的替代方法。
