Domashenko A D, Umanskiĭ S R
Radiobiologiia. 1984 Jan-Feb;24(1):76-9.
Using PAAG-electrophoresis and subsequent fluorography no essential disorders were detected in the biosynthesis of some protein fractions of the cytoplasm and nuclear sap of thymocytes during the first 2 h following irradiation of rats with a dose of 8 Gy. There was a decrease, progressing in time, in the rate of the synthesis of certain polypeptides. Inhibition of the biosynthesis of the proteins reacting with DNA was demonstrated by the chromatography, on DNA-containing columns, of labeled proteins of different fractions. The most pronounced changes were observed in the group of firmly bound proteins.
采用聚丙烯酰胺凝胶电泳及随后的荧光自显影技术,在给大鼠8戈瑞剂量照射后的最初2小时内,未检测到胸腺细胞胞质和核液中某些蛋白质组分的生物合成有实质性紊乱。某些多肽的合成速率随时间推移而下降。通过在含DNA柱上对不同组分的标记蛋白进行层析,证明了与DNA反应的蛋白质的生物合成受到抑制。在牢固结合蛋白组中观察到最明显的变化。