Kricka L J, Ogden S, Williams T M, Kant J
Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104.
J Biolumin Chemilumin. 1989 Jul-Sep;3(3):155-8. doi: 10.1002/bio.1170030309.
Frozen assay reagents have been used to reduce the rate of light emission from the rapid chemiluminescent acridinium ester and the bioluminescent firefly luciferase reactions. Melting of the assay reagent delays the initiation of the light emission, thus eliminating the need to initiate these rapid reactions by injection of the assay reagents in front of the photodetector.
冷冻检测试剂已被用于降低快速化学发光吖啶酯和生物发光萤火虫荧光素酶反应的发光速率。检测试剂的融化会延迟发光的起始,从而无需在光电探测器前通过注射检测试剂来启动这些快速反应。