Fozza Claudio, Barraqueddu Francesca, Corda Giovanna, Contini Salvatore, Virdis Patrizia, Dore Fausto, Bonfigli Silvana, Longinotti Maurizio
Hematology, Department of Clinical and Experimental Medicine, University of Sassari, Viale San Pietro 12, 07100 Sassari, Italy.
Hematology, Department of Clinical and Experimental Medicine, University of Sassari, Viale San Pietro 12, 07100 Sassari, Italy.
J Immunol Methods. 2017 Jan;440:1-11. doi: 10.1016/j.jim.2016.11.001. Epub 2016 Nov 5.
The T-cell receptor (TCR) is the key player within the so called immunological synapse and the analysis of its repertoire offers a picture of both versatility and wideness of the whole immune T-cell compartment. Among the different approaches applied to its study the so-called spectratyping identifies the pattern of the third complementarity determining region (CDR3) length distribution in each one of the beta variable (TRBV) subfamilies encoded by the corresponding genes. This technique consists in a CDR3 fragment analysis through capillary electrophoresis, performed after cell separation, RNA extraction and reverse transcriptase PCR. This review will run through the most relevant studies which have tried to dissect the TCR repertoire usage in patients with different immune-mediated and infective diseases as well as solid or haematologic malignancies.
T细胞受体（TCR）是所谓免疫突触中的关键参与者，对其库的分析展现了整个免疫T细胞区室的多样性和广泛性。在应用于其研究的不同方法中，所谓的谱型分析确定了由相应基因编码的每个β可变区（TRBV）亚家族中第三互补决定区（CDR3）长度分布的模式。该技术包括在细胞分离、RNA提取和逆转录酶PCR后，通过毛细管电泳进行CDR3片段分析。本综述将梳理那些试图剖析不同免疫介导性疾病、感染性疾病以及实体或血液系统恶性肿瘤患者TCR库使用情况的最相关研究。
