Weitman S D, Phelan A M, Lech J J, Lange D G
Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226.
Biochem Pharmacol. 1989 Sep 15;38(18):2949-55. doi: 10.1016/0006-2952(89)90002-6.
Propranolol-induced alterations of membrane structure were studied in rat erythrocytes using electron spin resonance techniques. Propranolol produced a concentration-dependent change in membrane fluidity in hydrophobic membrane regions, while producing virtually no change in hydrophilic membrane regions. The changes were associated with depth-dependent alterations in "apparent" phase-transition profiles and transition temperatures. The effects of propranolol on these membrane characteristics were similar to those produced by cholesterol. Propranolol fluidized erythrocyte membranes in a depth-specific fashion, by virtue of its association with the rigid phospholipid acyl chains and cholesterol sterol rings in the hydrophilic regions of the membrane, which produced distant perturbations within the hydrophilic regions of the membrane.
利用电子自旋共振技术，在大鼠红细胞中研究了普萘洛尔引起的膜结构变化。普萘洛尔使疏水膜区域的膜流动性产生浓度依赖性变化，而亲水膜区域几乎没有变化。这些变化与“表观”相变曲线和转变温度的深度依赖性改变有关。普萘洛尔对这些膜特性的影响与胆固醇产生的影响相似。普萘洛尔通过与膜亲水区域的刚性磷脂酰基链和胆固醇甾醇环结合，以深度特异性方式使红细胞膜流化，从而在膜的亲水区域内产生远距离扰动。
