Waage A, Brandtzaeg P, Halstensen A, Kierulf P, Espevik T
Institute of Cancer Research, University of Trondheim, Norway.
J Exp Med. 1989 Jan 1;169(1):333-8. doi: 10.1084/jem.169.1.333.
Serum samples from patients with meningococcal disease were examined for the presence of IL-6, TNF-alpha, and LPS. Median serum concentration of IL-6 was 1,000 times higher in patients with septic shock (189 ng/ml) than in patients with bacteriaemia, meningitis, or combined septic shock and meningitis. 11 of 21 patients with serum levels greater than 3.0 ng/ml died, whereas all 58 patients with serum levels at less than or equal to 3.0 ng/ml, survived. All four patients with serum IL-6 levels greater than 750 ng/ml, died. IL-1 was detected in serum from three patients who also had high serum levels of IL-6, TNF-alpha, and LPS, and rapidly fatal courses. IL-6 appeared to be released into serum later than TNF-alpha, and was detected in serum for up to 36 h. The half-life of IL-6 and TNF-alpha was calculated to be 103 +/- 27 min and 70 +/- 11 min, respectively. These data indicate that a complex pattern of cytokines exists in serum from patients with meningococcal septic shock, and that the release of IL-6 and IL-1, in addition to TNF-alpha, is associated with fatal outcome.
对患有脑膜炎球菌病患者的血清样本进行检测，以确定是否存在白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和脂多糖（LPS）。感染性休克患者血清中IL-6的中位浓度（189纳克/毫升）比菌血症、脑膜炎或合并感染性休克和脑膜炎的患者高1000倍。血清水平大于3.0纳克/毫升的21名患者中有11人死亡，而血清水平小于或等于3.0纳克/毫升的所有58名患者均存活。血清IL-6水平大于750纳克/毫升的所有4名患者均死亡。在3名血清IL-6、TNF-α和LPS水平也较高且病程迅速致命的患者血清中检测到白细胞介素-1（IL-1）。IL-6似乎比TNF-α释放到血清中的时间更晚，在血清中可检测到长达36小时。计算得出IL-6和TNF-α的半衰期分别为103±27分钟和70±11分钟。这些数据表明，脑膜炎球菌感染性休克患者血清中存在复杂的细胞因子模式，并且除TNF-α外，IL-6和IL-1的释放与致命结局相关。
