Waage A, Espevik T
Cell Research Laboratory, Institute of Cancer Research, University of Trondheim, Norway.
J Exp Med. 1988 Jun 1;167(6):1987-92. doi: 10.1084/jem.167.6.1987.
Human rIL-1 alpha and human rIL-1 beta were examined for their ability to potentiate the lethal and hypothermic effects of mouse rTNF-alpha in mice. The LD50 of rTNF-alpha was 1.5 micrograms/mouse, whereas the LD50 of rTNF-alpha was reduced to 0.4 micrograms/mouse and 0.5 micrograms/mouse when rTNF-alpha was administered in combination with a nonlethal dose of rIL-1 alpha or rIL-1 beta, respectively. A similar rTNF-alpha enhancing effect of the rIL-1 was observed on the temperature response. The results show that the rIL-1 markedly potentiate the effects of rTNF-alpha on lethality and temperature in mice, and support our suggestion that TNF-alpha and IL-1 may have synergistic lethal effect in human septic shock.
研究了人重组白细胞介素-1α(rIL-1α)和人重组白细胞介素-1β(rIL-1β)增强小鼠rTNF-α致死和降温作用的能力。rTNF-α的半数致死量(LD50)为1.5微克/只小鼠,而当rTNF-α分别与非致死剂量的rIL-1α或rIL-1β联合给药时,rTNF-α的LD50分别降至0.4微克/只小鼠和0.5微克/只小鼠。在温度反应方面也观察到了rIL-1对rTNF-α类似的增强作用。结果表明,rIL-1可显著增强rTNF-α对小鼠致死性和体温的影响,支持了我们提出的TNF-α和IL-1在人类脓毒症休克中可能具有协同致死作用的观点。