Estrada Norma, Velázquez Edwin, Rodríguez-Jaramillo Carmen, Ascencio Felipe
Programa Cátedras CONACyT, Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), 23090 La Paz, BCS, Mexico.
Laboratorio de Patogénesis Microbiana, Centro de Investigaciones Biológicas del Noroeste, S.C., 23090 La Paz, BCS, Mexico.
Int J Cell Biol. 2016;2016:9032181. doi: 10.1155/2016/9032181. Epub 2016 Oct 19.
Hemocytes represent one of the most important defense mechanisms against foreign material in Crustacea and are also involved in a variety of other physiological responses. Fluorescent lectin-binding assays and cytochemical reactions were used to identify specificity and distribution of carbohydrate moieties and presence of several hydrolytic enzymes, in hemocytes of whiteleg shrimp . Two general classes of circulating hemocytes (granular and agranular) exist in , which express carbohydrates residues for FITC-conjugated lectins WGA, LEA, and PNA; UEA and Con-A were not observed. Enzymatic studies indicated that acid phosphatase, nonspecific esterase, and specific esterases were present; alkaline phosphatase was not observed. The enzymes and carbohydrates are useful tools in hemocyte classification and cellular defense mechanism studies.
血细胞是甲壳类动物抵御外来物质的最重要防御机制之一，并且还参与多种其他生理反应。荧光凝集素结合试验和细胞化学反应被用于鉴定凡纳滨对虾血细胞中碳水化合物部分的特异性和分布以及几种水解酶的存在情况。凡纳滨对虾体内存在两大类循环血细胞（颗粒细胞和无颗粒细胞），它们表达针对异硫氰酸荧光素偶联凝集素WGA、LEA和PNA的碳水化合物残基；未观察到对UEA和伴刀豆球蛋白A的反应。酶学研究表明存在酸性磷酸酶、非特异性酯酶和特异性酯酶；未观察到碱性磷酸酶。这些酶和碳水化合物是血细胞分类和细胞防御机制研究中的有用工具。
