Martin Gary G, Graves Brenda L
Department of Biology, Occidental College, Los Angeles, California 90041.
J Morphol. 1985 Sep;185(3):339-348. doi: 10.1002/jmor.1051850306.
The structure of hemocytes from two species of penaeid shrimp was examined by light and electron (TEM) microscopy. Hemocytes from the two species are indistinguishable and are classified as either agranular, small-granule, or large-granule hemocytes. Agranular hemocytes are the smallest of the hemocytes, lack granules, compose only 5-10% of the circulating hemocytes, and are nonrefractile when examined by light microscopy. Small-granule hemocytes are the most abundant type of hemocyte (75% of all hemocytes), appear nonrefractile, and contain a variable number (1-40) of granules (0.4 μm diameter). Large-granule hemocytes compose 10-20% of the hemocytes. They are filled with granules (0.8 μm in diameter) that are highly refractile when examined by light microscopy and are electron-dense when examined by TEM. Our classification scheme is based solely on the absence or presence and relative size of granules. Features used by other researchers, such as cell size, shape, and staining properties, were not used because these features are subtle and/or subjective. The proposed classification is compared with schemes developed for other decapods, and its usefulness and limitations are discussed. This scheme will serve as a basis for further studies on the maturation and physiological function(s) or crustacean hemocytes.
通过光学显微镜和电子显微镜（透射电子显微镜，TEM）对两种对虾血细胞的结构进行了检查。这两种对虾的血细胞难以区分，可分为无颗粒血细胞、小颗粒血细胞或大颗粒血细胞。无颗粒血细胞是血细胞中最小的，没有颗粒，仅占循环血细胞的5%-10%，在光学显微镜下检查时不折射。小颗粒血细胞是最丰富的血细胞类型（占所有血细胞的75%），看起来不折射，含有数量可变（1-40个）的颗粒（直径0.4μm）。大颗粒血细胞占血细胞的10%-20%。它们充满了颗粒（直径0.8μm），在光学显微镜下检查时高度折射，在透射电子显微镜下检查时电子密度高。我们的分类方案仅基于颗粒的有无和相对大小。其他研究人员使用的特征，如细胞大小、形状和染色特性，未被采用，因为这些特征细微且/或主观。将所提出的分类与为其他十足目动物制定的分类方案进行了比较，并讨论了其有用性和局限性。该方案将作为进一步研究甲壳类动物血细胞成熟和生理功能的基础。
