Charoensup Rawiwan, Duangyod Thidarat, Phuneerub Pravaree, Singharachai Chatubhong
School of Health Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
J Adv Pharm Technol Res. 2016 Oct-Dec;7(4):134-138. doi: 10.4103/2231-4040.191421.
(Dennst.) Alston. () or Ma-khwaen is one of the medicinal plants in Thai traditional medicine. To investigate the pharmacognostic specifications and chemical constituents of fruits and seeds. Fruits and seeds of were collected from 15 sources throughout Thailand; then examined the pharmacognostic specification following WHO guideline of quality control method for medicinal plant materials. Microscopic determination of powders demonstrated fragment of mesocarp, fragment of brown vitta, oil glands, fragment of endocarp, and endosperm containing oil globule, trichome and pale brown stone cells. Stomatal index and pellucid dots in mm were 19.87 and 4.2 respectively. Physico-chemical parameters unveiled that loss on drying, water content, total ash, and acid-insoluble ash should be not >17.90%, 9.18%, 4.50%, and 0.60% of dried weight respectively; while ethanol, water, and hexane extractive values and volatile oil content should be not <2.24%, 2.27%, 1.57% and 9.63% of dried weight respectively. R values of thin-layer chromatographic fingerprint of fruits and seeds ethanolic extract were 0.38, 0.45, 0.90, and 0.97 detected ultraviolet (UV) light 254 nm, 0.30, 0.44, 0.67, and 0.77 detected UV light 366 nm, and 0.24, 0.73, 0.78, and 0.93 detected 10% sulfuric acid. There are three main chemical compounds in oil including limonene (43.63%), (+)-sabinene (16.72%), and terpinen-4-ol (10.95%). The result gained from pharmacognostic specifications and chemical fingerprints could be used as standardization data of fruits and seeds to apply or provide for guarantee of quality.
（丹斯特）阿尔斯顿。（）或马夸恩是泰国传统医学中的药用植物之一。为研究其果实和种子的生药学特征及化学成分。从泰国各地15个来源收集其果实和种子；然后按照世界卫生组织药用植物材料质量控制方法指南检查生药学特征。对粉末进行显微镜鉴定显示有中果皮碎片、棕色油管碎片、油腺、内果皮碎片以及含有油球、毛状体和浅棕色石细胞的胚乳。气孔指数和每毫米透明点分别为19.87和4.2。物理化学参数表明，干燥失重、水分含量、总灰分和酸不溶性灰分分别不应超过干燥重量的17.9０%、9.18%、4.50%和0.60%；而乙醇、水和己烷提取物值以及挥发油含量分别不应低于干燥重量的2.24%、2.27%、1.57%和9.63%。其果实和种子乙醇提取物的薄层色谱指纹图谱在254nm紫外光下检测到的Rf值为0.38、0.45、0.90和0.97，在366nm紫外光下检测到的Rf值为0.30、0.44、0.67和0.77，在10%硫酸下检测到的Rf值为0.24、0.73、0.78和0.93。其油中有三种主要化合物，包括柠檬烯（43.63%）、（+）-桧烯（16.72%）和萜品-4-醇（10.95%）。从生药学特征和化学指纹图谱获得的结果可作为其果实和种子的标准化数据，用于应用或提供质量保证。
