Pharmacognostic Specification, Chlorogenic Acid Content, and Antioxidant Activities of Flowering Bud.

BACKGROUND

Thunb. or Japanese Honeysuckle has been widely used in traditional medicine for antipyretic.

OBJECTIVE

To establish the pharmacognostic specification of flowering bud in Thailand and to determine its chlorogenic acid content and antioxidant activities.

MATERIALS AND METHODS

Dried flowering bud from 15 various herbal drugstores throughout Thailand were investigated for pharmacognostic specification. Their chlorogenic acid contents were quantitatively analyzed by thin layer chromatography (TLC) densitometry with winCATS software. The mobile phase for TLC development consisted of ethyl acetate: formic acid: acetic acid: water (10:1.1:1.1:2.6). Antioxidant activities were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric ion reducing antioxidant power assay, nitric oxide scavenging assay, and β-carotene bleaching assays.

RESULTS

Qualified flowering bud in Thailand was presented that the contents of loss on drying, total ash, acid-insoluble ash, and water should not be >10.11%, 6.59%, 1.14%, and 10.82% by weight, respectively. The ethanol and water soluble extractive values should not be < 16.46% and 28.88% by weight, respectively. Chlorogenic acid content in flowering bud was found to be 2.24 ± 0.50 g/100 g of crude drug. flowering bud showed DPPH and nitric oxide scavenging activities as well as reducing power property.

CONCLUSION

This pharmacognostic specification with special reference to the chlorogenic acid content can be used for quality control of flowering bud in Thailand. The potential antioxidant of this crude drug was demonstrated .

SUMMARY

Pharmacognostic specification of flowering bud in Thailand has been establishedThe chlorogenic acid content has been quantified by thin layer chromatography-densitometryThe ethanolic extract of flowering bud showed antioxidation potential, especially on reducing power property. TLC: Thin layer chromatography, DPPH: 2,2-diphenyl-1-picrylhydrazyl, FRAP: Ferric ion Reducing Antioxidant Power, WHO: World Health Organization, ICH: International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use; LOD: Limit of detection; LOQ: Limit of quantitation; BHT: Butylated hydroxytoluene; FeSO: Iron(II) sulfate; DMSO: Dimethyl sulfoxide; TPTZ: 2,4,6-tripyridyl-s-triazine.