Takahashi N, Kukita T, MacDonald B R, Bird A, Mundy G R, McManus L M, Miller M, Boyde A, Jones S J, Roodman G D
Veterans Administration Hospital, San Antonio, Texas.
J Clin Invest. 1989 Feb;83(2):543-50. doi: 10.1172/JCI113916.
Transplantation studies have suggested that peripheral blood mononuclear cells contain precursors for osteoclasts. Thus we tested the capacity of peripheral blood monocytes to form osteoclasts in long-term culture. We have reported previously that mononuclear cells from feline, baboon, and human marrow form osteoclast-like cells in long term cultures. Further, the formation of these cells is increased in response to bone resorption stimulatory agents such as PTH, interleukin 1, and transforming growth factor alpha. We now report that these cells show characteristic cytoplasmic contraction with calcitonin and form resorption lacunae when cultured on sperm whale dentine. Thus, these bone marrow-derived multinucleated cells fulfill the functional criteria for osteoclasts. Although cultured peripheral blood monocytes can be induced to form multinucleated cells with 1,25-dihydroxyvitamin D3, these cells did not show similar responses to the osteotropic factors as multinucleated cells formed in the bone marrow cultures multinucleated cells. These results indicate that osteoclasts or cells closely related to osteoclasts form in long-term human bone marrow cultures. In contrast, few mononuclear cells in the peripheral blood appear capable of forming osteoclasts under the culture conditions used in these experiments.
移植研究表明，外周血单个核细胞含有破骨细胞的前体细胞。因此，我们测试了外周血单核细胞在长期培养中形成破骨细胞的能力。我们之前报道过，来自猫、狒狒和人类骨髓的单个核细胞在长期培养中可形成破骨细胞样细胞。此外，这些细胞的形成会因骨吸收刺激剂（如甲状旁腺激素、白细胞介素1和转化生长因子α）而增加。我们现在报告，这些细胞在降钙素作用下表现出典型的细胞质收缩，并且在抹香鲸牙质上培养时会形成吸收陷窝。因此，这些源自骨髓的多核细胞符合破骨细胞的功能标准。尽管培养的外周血单核细胞可被1,25 - 二羟维生素D3诱导形成多核细胞，但这些细胞对促骨因子的反应与骨髓培养中形成的多核细胞不同。这些结果表明，破骨细胞或与破骨细胞密切相关的细胞在长期人类骨髓培养中形成。相比之下，在这些实验所用的培养条件下，外周血中几乎没有单核细胞能够形成破骨细胞。
