State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033 Changchun, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130033 Changchun, China.
State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033 Changchun, China.
Biosens Bioelectron. 2017 Jun 15;92:517-522. doi: 10.1016/j.bios.2016.10.070. Epub 2016 Oct 28.
Assay technologies capable of detecting biomarker concentrations in unprocessed whole blood samples are fundamental for applications in medical diagnostics. SERS nano-tags integrated fiber-optic biosensor (FOB) was realized for the first time for in situ immunoassay in whole blood. The reliability and sensitivity of this method rely, in a large extent, on the quality and properties of the SERS nano-tags. The constructed silica-coated Ag SERS nano-tags as labels were used in a rapid and specific in situ FOB immune sensor to detect alpha fetoprotein (AFP) in unprocessed blood samples. Preliminary results of in vivo and in situ dynamic observation of AFP of whole blood in wistar rat highlight the power of this new method.
用于检测未处理全血样本中生物标志物浓度的分析技术对于医学诊断应用至关重要。首次实现了基于表面增强拉曼散射（SERS）纳米标签的集成光纤生物传感器（FOB），用于全血中的原位免疫分析。该方法的可靠性和灵敏度在很大程度上取决于 SERS 纳米标签的质量和特性。构建的二氧化硅包覆的银 SERS 纳米标签作为标记物，用于快速、特异的原位 FOB 免疫传感器，以检测未经处理的血样中的甲胎蛋白（AFP）。该方法在活体和原位动态观察 Wistar 大鼠全血 AFP 方面的初步结果，突出了这种新方法的强大功能。
