基于近红外香豆素的荧光探针用于选择性检测水合肼及其在活细胞中的应用。
A near-infrared xanthene-based fluorescent probe for selective detection of hydrazine and its application in living cells.
机构信息
Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, PR China.
National Food Quality Supervision and Inspection Center (Shanghai), Shanghai Institute of Quality Inspection and Technical Research, Shanghai, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5;223:117344. doi: 10.1016/j.saa.2019.117344. Epub 2019 Jul 9.
Developing fluorescent probes for selective determination of the toxic and carcinogenic hydrazine are pretty significant. Herein, a rhodamine dye coupled to naphthalene was selected as a near-infrared fluorophore and acetyl group as a trigger unit for hydrazine sensing with a Stokes shifts of 62 nm. The probe showed about 77-fold NIR fluorescence enhancement in the presence of hydrazine. In addition, the detection limit was as low as 3.4 ppb, and the fluorescence intensity at 654 nm showed a satisfactory linearity with the concentration range of hydrazine from 0 to 120 μM. More importantly, the practical utility of probe has been successfully proved through the fluorescence bioimaging of hydrazine in living cells with low cytotoxicity and quantitative NH detection in environmental water samples.
开发用于选择性测定有毒和致癌肼的荧光探针具有重要意义。本文选择罗丹明染料与萘偶联作为近红外荧光团，以乙酰基作为肼传感的触发单元，斯托克斯位移为 62nm。探针在存在肼的情况下表现出约 77 倍的近红外荧光增强。此外，检测限低至 3.4ppb，在 654nm 处的荧光强度与肼的浓度范围 0 至 120μM 呈令人满意的线性关系。更重要的是，通过低细胞毒性的荧光生物成像和环境水样中定量 NH 的检测，成功证明了探针的实际应用。
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