College of Materials Science and Engineering, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, Nanshan District Key Lab for Biopolymers and Safety Evaluation, Shenzhen University, Shenzhen 518060, PR China; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, PR China.
College of Materials Science and Engineering, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, Nanshan District Key Lab for Biopolymers and Safety Evaluation, Shenzhen University, Shenzhen 518060, PR China.
Talanta. 2019 Apr 1;195:857-864. doi: 10.1016/j.talanta.2018.12.015. Epub 2018 Dec 10.
Hydrazine is an important industrial material yet highly toxic and extremely harmful to people's health when over-exposed in the environment, thus monitoring hydrazine is of great significance. In this work, a novel highly fluorescent fluorophore BQ-OH, based on hydroxyl- and benzo[d]oxazole-substituted quinoline structure, was synthesized and esterified with 4-biomobutyric acid to afford a fluorescent probe BQABr for the selective detection of hydrazine. The probe follows SN2(nucleophilic substitution)-cyclization sensing mechanism with remarkable response, excellent sensitivity and selectivity. Spectra experiments in aqueous solutions demonstrated that BQABr exhibited an excellent ratiometric fluorescence response toward hydrazine with two well separated emission bands before/after sensing reaction. Emission peak shifted over 130 nm from 387 nm to 521 nm, and unexpectedly outstanding ratio signal enhancement over 3000-fold was achieved. Due to the large spectra response, a very low detection limit of 5.8 nM (0.19 ppb) was obtained. Selectivity experiment was performed, showing BQABr had nearly no spectra response to other possible disturbing analytes. The probe-coated test papers were fabricated and successfully applied to detect gaseous hydrazine. Furthermore, potential application for the detection of hydrazine in both environmental samples and biological samples (living cells) has been demonstrated.
水合肼是一种重要的工业原料，但在环境中过度暴露时对人体健康极具毒性和危害性，因此对水合肼进行监测具有重要意义。在这项工作中，我们合成了一种基于羟基和苯并[d]恶唑取代喹啉结构的新型高荧光荧光团 BQ-OH，并将其与 4-生物丁二酸酯化，得到用于选择性检测水合肼的荧光探针 BQABr。该探针遵循 SN2（亲核取代）-环化感应机制，具有显著的响应、优异的灵敏度和选择性。在水溶液中的光谱实验表明，BQABr 对水合肼表现出出色的比率荧光响应，在感应反应前后具有两个分离良好的发射带。发射峰从 387nm 移动到 521nm，超过 130nm，并且出人意料地实现了超过 3000 倍的出色比值信号增强。由于光谱响应较大，检测限低至 5.8nM（0.19ppb）。选择性实验表明，BQABr 对其他可能存在的干扰分析物几乎没有光谱响应。我们制备了探针涂层测试纸，并成功用于检测气态水合肼。此外，还证明了该探针在环境样品和生物样品（活细胞）中检测水合肼的潜在应用。
