Liu Hang, Xie Linan, Wang Jing, Guo Qiannan, Yang Shengnan, Liang Pei, Wang Chengshu, Lin Min, Xu Yuquan, Zhang Liwen
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
Department of Entomology, China Agricultural University, Beijing 100083, P.R. China.
J Microbiol Biotechnol. 2017 Mar 28;27(3):439-449. doi: 10.4014/jmb.1606.06056.
infects a number of pest species and is known to produce insecticidal substances, such as the nonribosomal peptides (NRPs) beauvericin and bassianolide. However, most NRPs and their biological roles in remain undiscovered. To identify NRPs that potentially contribute to pathogenesis, the 21 predicted NRP synthetases (NRPSs) or NRPS-like proteins of ARSEF 2860 were primarily ranked into three functional groups: basic metabolism (7 NRPSs), pathogenicity (12 NRPSs), and unknown function (2 NRPSs). Based on the transcript levels during in vivo growth on diamondback moth ( (Linnaeus)), half of the Group II NRPSs were likely to be involved in infection. Given that the metabolites biosynthesized by these NRPSs remain to be determined, our result underlines the importance of the NRPSome in fungal pathogenesis, and will serve as a guide for future genomic mining projects to discover functionally essential and structurally diverse NRPs in fungal genomes.
它能感染多种害虫，并已知能产生杀虫物质，如非核糖体肽（NRP）白僵菌素和白僵菌内酯。然而，大多数NRP及其在……中的生物学作用仍未被发现。为了鉴定可能对致病机制有贡献的NRP，ARSEF 2860的21种预测的NRP合成酶（NRPS）或NRPS样蛋白主要被分为三个功能组：基本代谢（7种NRPS）、致病性（12种NRPS）和未知功能（2种NRPS）。基于在小菜蛾（（林奈））体内生长期间的转录水平，第二组NRPS中的一半可能参与感染。鉴于这些NRPS生物合成的代谢物仍有待确定，我们的结果强调了NRPSome在真菌致病机制中的重要性，并将为未来的基因组挖掘项目提供指导，以在真菌基因组中发现功能上必不可少且结构多样的NRP。
