柳属（杨柳科）线粒体基因组完整序列的组装与分析。
Assembly and analysis of the complete L. (Salicaceae) mitochondrial genome sequence.
作者信息
Wei Suyun, Wang Xuelin, Bi Changwei, Xu Yiqing, Wu Dongyang, Ye Ning
机构信息
College of Forestry, Nanjing Forestry University, Nanjing, 210037 Jiangsu China ; The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, 210037 Jiangsu China ; College of Information Science and Technology, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
College of Information Science and Technology, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
出版信息
Springerplus. 2016 Oct 28;5(1):1894. doi: 10.1186/s40064-016-3521-6. eCollection 2016.
Plant mitochondrial (mt) genomes possess several complex features, including a variable size, a dynamic genome structure, and complicated patterns of gene loss and gain throughout evolutionary history. Studies of plant mt genomes can, therefore, provide unique insights into organelle evolution. We assembled the complete L. mt genome by screening genomic sequence reads generated by a Roche-454 pyrosequencing platform. The pseudo-molecule obtained has a typical circular structure 598,970 bp long, with an overall GC content of 55.06%. The mt genome contains 52 genes: 31 protein-coding, 18 tRNAs, and three rRNAs. Eighteen tandem repeats and 404 microsatellites are distributed unevenly throughout the mt genome. A phylogenetic tree of 23 representative terrestrial plants strongly supports inclusion in the Malpighiales clade. Our analysis contributes toward understanding the organization and evolution of organelle genomes in Salicaceae species.
植物线粒体（mt）基因组具有几个复杂的特征，包括大小可变、基因组结构动态变化，以及在整个进化历史中基因丢失和获得的复杂模式。因此，对植物mt基因组的研究可以为细胞器进化提供独特的见解。我们通过筛选由罗氏454焦磷酸测序平台生成的基因组序列读数，组装了完整的L. mt基因组。获得的假分子具有典型的环状结构，长度为598,970 bp，总体GC含量为55.06%。该mt基因组包含52个基因：31个蛋白质编码基因、18个tRNA和3个rRNA。18个串联重复序列和404个微卫星在整个mt基因组中分布不均。23种代表性陆生植物的系统发育树有力地支持了将其纳入金虎尾目分支。我们的分析有助于理解杨柳科物种细胞器基因组的组织和进化。
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