Biondi A, Rossi V, Bassan R, Barbui T, Bettoni S, Sironi M, Mantovani A, Rambaldi A
Clinica Pediatrica Università di Milano, Ospedale S. Gerardo, Italy.
Blood. 1989 Apr;73(5):1279-84.
Interleukin-6 (IL-6) is a pleiotropic lymphokine active as a growth factor on B-cell hybridomas and plasmacytomas and found to be identical with B-cell stimulatory factor 2, interferon beta 2, 26-Kd protein, and hepatocytes stimulating factor. IL-6 gene expression was investigated in fresh human chronic lymphocytic leukemia (B-CLL) and in acute lymphoblastic leukemia (ALL) by Northern blot analysis using a specific cDNA probe. 1.3-kb IL-6 transcript was found in six out of 11 B-CLL patients, while no hybridization was observed in ten cases of ALL of both T- and B-cell origin. The constitutive expression of IL-6 transcripts was associated with production of a biologically active protein as determined by using the IL-6-dependent 7TD1 cell line. It remains to be elucidated whether IL-6 gene expression is indeed important in the regulation of B-CLL growth or in its clinical manifestation.
白细胞介素-6（IL-6）是一种多效性淋巴因子，作为B细胞杂交瘤和浆细胞瘤的生长因子具有活性，并且被发现与B细胞刺激因子2、干扰素β2、26-Kd蛋白以及肝细胞刺激因子相同。使用特异性cDNA探针，通过Northern印迹分析在新鲜的人类慢性淋巴细胞白血病（B-CLL）和急性淋巴细胞白血病（ALL）中研究了IL-6基因表达。在11例B-CLL患者中的6例中发现了1.3-kb的IL-6转录本，而在10例T细胞和B细胞来源的ALL病例中均未观察到杂交信号。通过使用依赖IL-6的7TD1细胞系确定，IL-6转录本的组成性表达与生物活性蛋白的产生相关。IL-6基因表达在B-CLL生长调节或其临床表现中是否确实重要，仍有待阐明。
