Wang Junfang, Wang Shuqin, Liu Guotian, Edwards Everard J, Duan Wei, Li Shaohua, Wang Lijun
Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of SciencesBeijing, China; Viticulture and Enology Research Center, Institute of Agro-food Science and Technology, Shandong Academy of Agricultural SciencesJinan, China.
Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of SciencesBeijing, China; College of Life Sciences, University of Chinese Academy of SciencesBeijing, China.
Front Plant Sci. 2016 Nov 3;7:1605. doi: 10.3389/fpls.2016.01605. eCollection 2016.
Resveratrols are polyphenolic secondary metabolites that can benefit human health, and only occur in a few plant families including Vitaceae. It has been reported that abscisic acid (ABA) can induce veraison (the onset of grape berry ripening) and may induce the accumulation of resveratrol in berry skin. However, the relationships between ABA, veraison, the accumulation of anthocyanins and the accumulation of resveratrol in the berry are poorly understood. This study attempted to answer this question through an investigation of the effect of applied ABA and fluridone (a synthetic inhibitor of ABA) on the biosynthesis and accumulation of ABA, anthocyanin, and resveratrol in Beihong ( × ) berry skin. Under natural conditions, resveratrol concentration was very low before 91 DAA (days after anthesis), i.e., 2 weeks after veraison, however, it increased sharply from this point to 126 DAA (maturity). Exogenous ABA applications all resulted in an increase in berry skin ABA and anthocyanin concentration, irrespective of the developmental stage at which the treatment occurred (20 and 10 days pre-veraison, veraison or 7 days post-veraison), thereby advancing veraison. In contrast, resveratrol concentration increased only when ABA was applied at 10 days pre-veraison or at veraison. As a result, the accumulation of resveratrol was associated with veraison in grape berry skin and this accumulation, together with that of anthocyanins, was associated with ABA concentration. The response of resveratrol biosynthesis in the berry skin to manipulation of ABA varied during berry development and was less sensitive to ABA than the response of anthocyanin biosynthesis.
白藜芦醇是一类对人体健康有益的多酚类次生代谢产物，仅在包括葡萄科在内的少数植物科中存在。据报道，脱落酸（ABA）可诱导葡萄转色（葡萄浆果成熟的开始），并可能诱导浆果表皮中白藜芦醇的积累。然而，ABA、转色、花青素积累与浆果中白藜芦醇积累之间的关系尚不清楚。本研究试图通过研究外源ABA和氟啶酮（一种ABA合成抑制剂）对北红葡萄浆果表皮中ABA、花青素和白藜芦醇生物合成及积累的影响来回答这个问题。在自然条件下，在盛花后天数（DAA）91天之前，即转色后2周，白藜芦醇浓度非常低，然而，从这一点到盛花后天数126天（成熟），其浓度急剧增加。外源ABA处理均导致浆果表皮ABA和花青素浓度增加，无论处理发生在哪个发育阶段（转色前20天和10天、转色期或转色后7天），从而使转色提前。相比之下，只有在转色前10天或转色期施用ABA时，白藜芦醇浓度才会增加。因此，葡萄浆果表皮中白藜芦醇的积累与转色有关，这种积累与花青素的积累一起与ABA浓度有关。浆果表皮中白藜芦醇生物合成对ABA调控的反应在浆果发育过程中有所不同，并且比花青素生物合成对ABA的反应更不敏感。
