Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan,430030, China.
Molecular Imaging Program at Stanford (MIPS), Bio-X Program, Department of Radiology, Stanford University, California, 94305-5344.
Nanoscale. 2016 Dec 1;8(47):19644-19653. doi: 10.1039/c6nr07298d.
Grafting a robust organic shell around inorganic nanoparticles can optimize their colloidal features to dramatically improve their physicochemical properties. Here, we have developed a polymer coating procedure for providing colloidal stability to the nanoparticles and, more importantly, for applying a fast, facile fluorine-18 labeling of iron oxide nanoparticles (IONPs) for positron emission tomography (PET)/magnetic resonance (MR) dual-modality imaging. The structure of the amphiphilic polymer is based on a backbone of polyacrylic acid, conjugated with multiple oleylamines to form a comb-like branched structure. The dense polymer shell provides high colloidal stability to the IONPs against harsh conditions such as high temperature, low pH value, and high ion strength. By incorporating a 1,4,7-triazacyclononane (NOTA) chelator to the comb-like amphiphilic polymer for the chelation of aluminum fluoride ions, we applied a one-step radiolabeling approach for a fast, facile radiofluorination of magnetic nanoparticles. The new strategy can significantly reduce the procedure time and radiation exposure. The PET/MR dual modality imaging was successfully achieved in living subjects by using F labeled magnetic nanoparticles.
在无机纳米粒子周围嫁接一个强健的有机壳，可以优化其胶体特性，从而显著改善其物理化学性质。在这里，我们开发了一种聚合物涂层程序，为纳米粒子提供胶体稳定性，更重要的是，为氧化铁纳米粒子（IONPs）进行正电子发射断层扫描（PET）/磁共振（MR）双模式成像提供快速、简便的氟-18 标记。两亲聚合物的结构基于聚丙烯酸的骨架，与多个油胺结合形成梳状分支结构。密集的聚合物壳为 IONPs 提供了高度的胶体稳定性，使其能够耐受高温、低 pH 值和高离子强度等苛刻条件。通过将 1,4,7-三氮杂环壬烷（NOTA）螯合剂结合到梳状两亲聚合物中，用于铝氟化物离子的螯合，我们应用了一种一步放射性标记方法，实现了磁性纳米粒子的快速、简便的放射性氟标记。新策略可以显著减少程序时间和辐射暴露。通过使用 F 标记的磁性纳米粒子，在活体动物中成功实现了 PET/MR 双模成像。
