Northwest Metabolomics Research Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, 98109, USA.
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Nanchang, Jiangxi Province, 330013, China.
Angew Chem Int Ed Engl. 2016 Dec 12;55(50):15646-15650. doi: 10.1002/anie.201609236. Epub 2016 Nov 15.
The balance between metabolism and biomass is very important in biological systems; however, to date there has been no quantitative method to characterize the balance. In this methodological study, we propose to use the distribution of amino acids in different domains to investigate this balance. It is well known that endogenous or exogenous amino acids in a biological system are either metabolized or incorporated into free amino acids (FAAs) or proteome amino acids (PAAs). Using glycine (Gly) as an example, we demonstrate a novel method to accurately determine the amounts of amino acids in various domains using serum, urine, and cell samples. As expected, serum and urine had very different distributions of FAA- and PAA-Gly. Using Tet21N human neuroblastoma cells, we also found that Myc(oncogene)-induced metabolic reprogramming included a higher rate of metabolizing Gly, which provides additional evidence that the metabolism of proliferating cells is adapted to facilitate producing new cells. It is therefore anticipated that our method will be very valuable for further studies of the metabolism and biomass balance that will lead to a better understanding of human cancers.
代谢与生物量平衡在生物系统中非常重要；然而，迄今为止，还没有一种定量方法来描述这种平衡。在这项方法学研究中，我们提出使用氨基酸在不同领域的分布来研究这种平衡。众所周知，生物系统中的内源性或外源性氨基酸要么被代谢，要么被掺入游离氨基酸（FAAs）或蛋白质组氨基酸（PAAs）中。本文以甘氨酸（Gly）为例，展示了一种新方法，可以准确测定血清、尿液和细胞样本中各种氨基酸的含量。不出所料，血清和尿液中 FAA-Gly 和 PAA-Gly 的分布差异很大。使用 Tet21N 人神经母细胞瘤细胞，我们还发现 Myc（致癌基因）诱导的代谢重编程包括更高的甘氨酸代谢率，这为增殖细胞的代谢适应性提供了更多证据，以促进新细胞的产生。因此，我们预计我们的方法将非常有助于进一步研究代谢和生物量平衡，从而更好地理解人类癌症。
