Sakuma H, Nakagawa T, Maeda H, Nakamura K, Takeda K, Hirano T, Yamaguchi N
Nihon Igaku Hoshasen Gakkai Zasshi. 1989 Jan 25;49(1):62-5.
Effective hepatic blood flow (EHBF) was measured from an uptake constant using single blood sampling and 99mTc-PMT hepatobiliary SPECT data. After intravenous injection of 3 mCi (111 MBq) of 99mTc-PMT, serial 1 min SPECT data were obtained for 7 minutes. A time activity curve (TAC) over the heart, that was normalized with the 5 minutes venous sample concentration (%/dose/ml), was used as a blood clearance curve (B(t]. And a TAC of the whole liver, that was normalized with the injected dose of 99mTc-PMT (%/dose), was used as a hepatogram (L(t]. An uptake constant representing EHBF, was estimated from the Rutland's method L. (t)/B(t) was plotted against integral of otB(t)dt/B(t), and the slope of the least square fitted straight line was determined as the uptake constant. In 16 cases, significant correlation was obtained between the 99mTc-PMT hepatic uptake at 5 minutes and the EHBF estimated from the blood clearance (r = 0.85, p less than 0.001). While there was a much better correlation between the EHBF from uptake constant and the EHBF from blood clearance (r = 0.95, p less than 0.001). In conclusion, this analysis for uptake constant of 99mTc-PMT SPECT data enables us to estimate EHBF with single venous sampling and in relatively short acquisition time. This method is thought to be very valuable in clinical practice.
采用单次采血和99mTc-PMT肝胆SPECT数据，根据摄取常数测量有效肝血流量（EHBF）。静脉注射3mCi（111MBq）的99mTc-PMT后，连续7分钟获取每分钟的SPECT数据。以心脏的时间-活性曲线（TAC），用5分钟静脉血样本浓度（%/剂量/毫升）进行归一化，作为血液清除曲线（B(t)）。以整个肝脏的TAC，用注射的99mTc-PMT剂量（%/剂量）进行归一化，作为肝脏影像（L(t)）。根据拉特兰方法估算代表EHBF的摄取常数，将L(t)/B(t)对αB(t)dt/B(t)的积分作图，将最小二乘拟合直线的斜率确定为摄取常数。在16例患者中，5分钟时99mTc-PMT肝脏摄取与通过血液清除估算的EHBF之间存在显著相关性（r = 0.85，p < 0.001）。而通过摄取常数得到的EHBF与通过血液清除得到的EHBF之间的相关性更好（r = 0.95，p < 0.001）。总之，对99mTc-PMT SPECT数据摄取常数的这种分析，使我们能够通过单次静脉采血和相对较短的采集时间来估算EHBF。该方法在临床实践中被认为非常有价值。
