用氢氧化钙糊剂对感染两种专性厌氧菌和一种兼性厌氧菌的牙本质小管进行消毒。

Disinfection by calcium hydroxide pastes of dentinal tubules infected with two obligate and one facultative anaerobic bacteria.

作者信息

Siqueira J F, de Uzeda M

机构信息

Oral Microbiology Laboratory, Institute of Microbiology, Federal University of Rio de Janeiro, Brazil.

出版信息

J Endod. 1996 Dec;22(12):674-6. doi: 10.1016/S0099-2399(96)80062-8.

DOI:10.1016/S0099-2399(96)80062-8

PMID:9220753

Abstract

Bovine dentine cylinders were experimentally infected with Actinomyces israelii, Fusobacterium nucleatum, or Enterococcus faecalis. The latter is a facultative anaerobic bacteria and the others are obligate anaerobes commonly found in endodontic infections. The infected specimens were exposed to pastes of calcium hydroxide mixed with saline solution or camphorated paramonochlorophenol for periods of 1 h, 1 day, and 1 week. The viability of bacteria after these exposure times was evaluated by specimen incubation in culture medium to compare the effectiveness of the pastes in disinfecting dentinal tubules. The calcium hydroxide/camphorated paramonochlorophenol paste effectively killed bacteria in the tubules after a 1-h period of exposure, except for E. faecalis that required one day of exposure. In contrast, the calcium hydroxide/saline paste was ineffective against E. faecalis and F. nucleatum even after 1 week of exposure. The results showed that camphorated paramonochlorophenol increased the antibacterial effects of calcium hydroxide.

摘要

牛牙本质圆柱体被用以色列放线菌、具核梭杆菌或粪肠球菌进行实验性感染。后者是兼性厌氧菌，其他的是通常在牙髓感染中发现的专性厌氧菌。将感染的标本暴露于与盐溶液或樟脑对氯苯酚混合的氢氧化钙糊剂中1小时、1天和1周。通过在培养基中孵育标本评估这些暴露时间后细菌的活力，以比较糊剂对牙本质小管消毒的有效性。氢氧化钙/樟脑对氯苯酚糊剂在暴露1小时后有效杀死了小管中的细菌，但粪肠球菌需要暴露一天。相比之下，即使暴露1周后，氢氧化钙/盐糊剂对粪肠球菌和具核梭杆菌也无效。结果表明，樟脑对氯苯酚增强了氢氧化钙的抗菌作用。