Dort Junio, Leblanc Nadine, Bryl Piotr, Fortin Marie-Gil, Carbonneau Marie-Elise, Lavigne Charles, Jacques Hélène
School of Nutrition, Laval University, Quebec City, QC, Canada G1V 0A6; Institute of Nutraceuticals and Functional Foods, Laval University, Quebec City, QC, Canada G1V 0A6.
Merinov, Quebec Fisheries and Aquaculture Innovation Centre, 96 montée de Sandy Beach, Office 1.07, Gaspé, QC, Canada G4X 2V6.
Biomed Res Int. 2016;2016:5214561. doi: 10.1155/2016/5214561. Epub 2016 Oct 27.
This study was designed to determine whether marine-derived proteins other than cod could have beneficial effects on inflammation following muscle injury. Macrophage and neutrophil densities were measured from bupivacaine-injured tibialis anterior muscle of rats fed isoenergetic diets containing either shrimp hydrolysate (Shr), casein hydrolysate (CaH), or whole casein (Ca). In this study, Shr reduced ED-macrophages at day 2 ( = 0.013), day 5 ( = 0.006), and day 14 after injury ( = 0.038) compared with Ca, indicating faster resolution of inflammation in Shr. Except for day 2 after injury where Shr led to lower ED-macrophages compared with CaH ( = 0.006), both Shr and CaH responded similarly at days 5, 14, and 28 after injury. This findings suggest that beneficial effects of Shr on ED-cells might be related to generation of anti-inflammatory peptides through the hydrolysis process, in addition to its high content of anti-inflammatory amino acids. However, while increasing myofiber cross-sectional area in noninjured muscles compared with both Ca and CaH, Shr failed to have a positive effect in corresponding injured muscles. These data indicate that shrimp hydrolysate can facilitate resolution of inflammation after muscle injury mainly through modulating proinflammatory macrophage accumulation but have less effect on optimal recovery in terms of muscle mass and fiber size.
本研究旨在确定除鳕鱼之外的海洋来源蛋白质是否对肌肉损伤后的炎症具有有益作用。在给大鼠喂食含虾水解物（Shr）、酪蛋白水解物（CaH）或全酪蛋白（Ca）的等能量饮食后，测量布比卡因损伤的胫前肌中的巨噬细胞和中性粒细胞密度。在本研究中，与Ca相比，Shr在损伤后第2天（P = 0.013）、第5天（P = 0.006）和第14天（P = 0.038）减少了ED - 巨噬细胞，表明Shr中炎症消退更快。除了损伤后第2天Shr导致的ED - 巨噬细胞比CaH少（P = 0.006）外，Shr和CaH在损伤后第5天、第14天和第28天的反应相似。这一发现表明，Shr对ED细胞的有益作用可能与其通过水解过程产生抗炎肽有关，此外还与其高含量的抗炎氨基酸有关。然而，与Ca和CaH相比，Shr虽然在未受伤的肌肉中增加了肌纤维横截面积，但在相应的受伤肌肉中未能产生积极作用。这些数据表明，虾水解物主要通过调节促炎巨噬细胞的积累来促进肌肉损伤后的炎症消退，但在肌肉质量和纤维大小方面对最佳恢复的作用较小。
