Exercise Biochemistry and Physiology Laboratory, Postgraduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
Cell Biochem Funct. 2011 Jan-Feb;29(1):43-9. doi: 10.1002/cbf.1716. Epub 2010 Dec 27.
Infrequent exercise, typically involving eccentric actions, has been shown to cause oxidative stress and to damage muscle tissue. High taurine levels are present in skeletal muscle and may play a role in cellular defences against free radical-mediated damage. This study investigates the effects of taurine supplementation on oxidative stress biomarkers after eccentric exercise (EE). Twenty-four male rats were divided into the following groups (n = 6): control; EE; EE plus taurine (EE + Taurine); EE plus saline (EE + Saline). Taurine was administered as a 1-ml 300 mg kg(-1) per body weight (BW) day(-1) solution in water by gavage, for 15 consecutive days. Starting on the 14th day of supplementation, the animals were submitted to one 90-min downhill run session and constant velocity of 1·0 km h(-1) . Forty-eight hours after the exercise session, the animals were killed and the quadriceps muscles were surgically removed. Production of superoxide anion, creatine kinase (CK) levels, lipoperoxidation, carbonylation, total thiol content and antioxidant enzyme were analysed. Taurine supplementation was found to decrease superoxide radical production, CK, lipoperoxidation and carbonylation levels and increased total thiol content in skeletal muscle, but it did not affect antioxidant enzyme activity after EE. The present study suggests that taurine affects skeletal muscle contraction by decreasing oxidative stress, in association with decreased superoxide radical production.
不频繁的运动，通常涉及离心运动，已被证明会引起氧化应激并损伤肌肉组织。高牛磺酸水平存在于骨骼肌中，可能在细胞防御自由基介导的损伤中发挥作用。本研究调查了牛磺酸补充对离心运动（EE）后氧化应激生物标志物的影响。24 只雄性大鼠分为以下几组（n=6）：对照组；EE 组；EE 加牛磺酸（EE+牛磺酸）组；EE 加盐水（EE+盐水）组。牛磺酸通过灌胃以 1 毫升 300mg/kg 体重（BW）/天的 300mg/kg BW 溶液给药，连续 15 天。在补充的第 14 天，动物开始进行 90 分钟下坡跑步，速度恒定为 1.0km/h。运动后 48 小时，处死动物并手术切除股四头肌。分析超氧阴离子、肌酸激酶（CK）水平、脂质过氧化、羰基化、总巯基含量和抗氧化酶的产生。牛磺酸补充可降低骨骼肌中超氧阴离子自由基的产生、CK、脂质过氧化和羰基化水平，并增加总巯基含量，但对 EE 后抗氧化酶活性没有影响。本研究表明，牛磺酸通过减少超氧自由基的产生来影响骨骼肌收缩，从而减轻氧化应激。
