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在具有多处差异的DNA链之间的异源双链体中，错配和未配对碱基附近匹配的胞嘧啶和胸腺嘧啶碱基的化学反应性。

Chemical reactivity of matched cytosine and thymine bases near mismatched and unmatched bases in a heteroduplex between DNA strands with multiple differences.

作者信息

Cotton R G, Campbell R D

机构信息

Murdoch Institute, Royal Children's Hospital, Melbourne, Australia.

出版信息

Nucleic Acids Res. 1989 Jun 12;17(11):4223-33. doi: 10.1093/nar/17.11.4223.

DOI:10.1093/nar/17.11.4223
PMID:2787020
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC317931/
Abstract

The chemical reactivity of matched T and C bases to osmium tetroxide and hydroxylamine near mismatched and unmatched bases in a heteroduplex between two strands of DNA with multiple differences was examined. Data was available for matched bases one or two positions away from 24 mismatches. Reactive bases were found near 16 of the mismatches and were usually one or two bases away. This reactivity is consistent with structural studies indicating perturbation of the duplex around mismatches and will allow another mode of study of the effect of mismatches. The reactivity of these bases was found not to be strongly correlated with mismatch type or GC basepair content of the basepairs around the mismatches. Extra reactivity may have been promoted by the presence of either T or C in the mismatch allowing increased reactivity of nearby T or C. The utility of the phenomenon for the detection of mutations is discussed. Unmatched bases in the heteroduplex also gives rise to reactive matched bases nearby.

摘要

研究了在具有多个差异的两条DNA链之间的异源双链体中，匹配的T和C碱基与四氧化锇和羟胺在错配和不匹配碱基附近的化学反应性。有关于距离24个错配位点一或两个位置的匹配碱基的数据。在16个错配位点附近发现了反应性碱基，通常距离错配位点一或两个碱基。这种反应性与结构研究一致，表明错配周围双链体受到扰动，并且将允许对错配效应进行另一种研究模式。发现这些碱基的反应性与错配类型或错配周围碱基对的GC碱基对含量没有强烈相关性。错配中T或C的存在可能促进了额外的反应性，从而增加了附近T或C的反应性。讨论了该现象在检测突变方面的实用性。异源双链体中的不匹配碱基也会在附近产生反应性匹配碱基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259f/317931/1dea9662436a/nar00128-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259f/317931/1dea9662436a/nar00128-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/259f/317931/1dea9662436a/nar00128-0219-a.jpg

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