碱基-碱基错配。dCA3XA3G + dCT3YT3G（X、Y = A、C、G、T）双螺旋形成的热力学
Base-base mismatches. Thermodynamics of double helix formation for dCA3XA3G + dCT3YT3G (X, Y = A,C,G,T).
作者信息
Aboul-ela F, Koh D, Tinoco I, Martin F H
出版信息
Nucleic Acids Res. 1985 Jul 11;13(13):4811-24. doi: 10.1093/nar/13.13.4811.
Abstract
Thermodynamic parameters for double strand formation have been measured for the sixteen double helices of the sequence dCA3XA3G.dCT3YT3G, with each of the bases A, C, G and T at the positions labelled X and Y. The results are analyzed in terms of nearest-neighbors and are compared with thermodynamic parameters for RNA secondary structure. At room temperature the sequence (Formula: see text) is more stable than (Formula: see text) and is similar in stability to (Formula: see text) and (Formula: see text) are least stable. At higher temperatures the sequences containing a G.C base pair become more stable than those containing only A.T. All molecules containing mismatches are destabilized with respect to those with only Watson-Crick pairing, but there is a wide range of destabilization. At room temperature the most stable mismatches are those containing guanine (G.T, G.G, G.A); the least stable contain cytosine (C.A, C.C). At higher temperatures pyrimidine-pyrimidine mismatches become the least stable.
摘要
已对序列为dCA3XA3G.dCT3YT3G的16个双螺旋进行了双链形成的热力学参数测定，其中碱基A、C、G和T分别位于标记为X和Y的位置。根据最近邻对结果进行了分析，并与RNA二级结构的热力学参数进行了比较。在室温下，序列（公式：见正文）比（公式：见正文）更稳定，并且与（公式：见正文）稳定性相似，而（公式：见正文）最不稳定。在较高温度下，含有G.C碱基对的序列比仅含有A.T的序列更稳定。所有含有错配的分子相对于仅具有沃森-克里克配对的分子都不稳定，但不稳定程度范围很广。在室温下，最稳定的错配是那些含有鸟嘌呤的（G.T、G.G、G.A）；最不稳定的含有胞嘧啶（C.A、C.C）。在较高温度下，嘧啶-嘧啶错配变得最不稳定。
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