Liang G, Gannett P, Gold B
Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha 68198-6805.
Nucleic Acids Res. 1995 Feb 25;23(4):713-9. doi: 10.1093/nar/23.4.713.
2-Hydroperoxytetrahydrofuran (THF-OOH) can be employed to sequence cytosine (C) and to probe for non-canonical DNA structures involving C. Using 32P-labeled oligomers and a DNA restriction fragment, it is demonstrated that THF-OOH has a strong preference for Cs in single-stranded (s-s) DNA regions, and in bulges, loops and mismatches. The reactivity of C is diminished below pH 6.0, but is not affected by substitution of 5-methylcytosine. To demonstrate the utility of the reagent, it is directly compared to methoxylamine and chloroacetaldehyde, two other reagents commonly used to chemically probe C residues in non-Watson-Crick DNA structures.
2-氢过氧化四氢呋喃（THF-OOH）可用于对胞嘧啶（C）进行测序，并探测涉及C的非经典DNA结构。使用32P标记的寡聚物和DNA限制片段，结果表明THF-OOH对单链（s-s）DNA区域、凸起、环和错配中的C具有强烈偏好。C的反应活性在pH 6.0以下会降低，但不受5-甲基胞嘧啶取代的影响。为了证明该试剂的实用性，将其与甲氧基胺和氯乙醛直接进行比较，后两者是常用于化学探测非沃森-克里克DNA结构中C残基的另外两种试剂。
