Abiko T, Sekino H
Chem Pharm Bull (Tokyo). 1989 Feb;37(2):391-6. doi: 10.1248/cpb.37.391.
[Glu34]human splenin (hSP) was synthesized in a conventional manner by assembling ten peptide fragments followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole (molar ratio, 1:1) in trifluoroacetic acid in the presence of m-cresol and dimethylselenide. Finally, the deprotected peptide was incubated with dithiothreitol to reduce sulfoxide on the methionine side chain. Incubation of peripheral lymphocytes isolated from uremic patients with the synthetic [Glu34]hSP showed an enhancing effect on the reduced B-lymphocytes, but synthetic human thymopoietin (hTP) had no effect under the same conditions.
[Glu34]人脾脏素（hSP）通过组装十个肽片段以常规方式合成，随后在间甲酚和二甲基硒存在下，于三氟乙酸中用1 M三氟甲磺酸 - 苯甲硫醚（摩尔比1:1）进行脱保护。最后，将脱保护的肽与二硫苏糖醇一起孵育以还原甲硫氨酸侧链上的亚砜。用合成的[Glu34]hSP孵育人尿毒症患者分离出的外周淋巴细胞，对减少的B淋巴细胞有增强作用，但在相同条件下合成的人胸腺生成素（hTP）没有作用。
