胆汁提取物对大鼠烧伤创面愈合的影响。

The effect of gall extract on burn wound healing in rat.

作者信息

Haghdoost Faraidoon, Baradaran Mahdavi Mohammad Mehdi, Zolfaghari Behzad, Sanei Mohammad Hossein, Najafi Somaye, Zandifar Alireza, Manouchehri Navid, Javanmard Shaghayegh Haghjooy

机构信息

Medical Students' Research Center, Isfahan University of Medical Sciences, Isfahan, Iran; Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.

Medical Students' Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Iran J Basic Med Sci. 2016 Oct;19(10):1144-1150.

PMID:27872712

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5110664/

Abstract

OBJECTIVES

The aim of the present study was to evaluate the effect of galls extract on the rat skin burn wound healing.

MATERIALS AND METHODS

Ethanol extract of the galls of was used to treat the induced burn wounds on the back of 32 Wistar rats divided into 4 groups. The groups were treated by placebo, 1%, 2% and 4% concentration gall extract gels for 14 days and the efficacy of treatment was assessed based on reduction of burn wound area, as well as histological and molecular characteristics.

RESULTS

The mean wound surface in the 14 day, in all groups treated by gall extracts were larger than control group and the differences were statistically significant (=0.043). The mean histological wound healing scores were not statistically different. Analysis of nitric oxide and platelet derived growth factor concentration in wound fluids in the 5 day of study showed that there was not any significant difference between groups (=0.468 and 0.312 respectively). Fibroblast growth factor (bFGF) concentration in the wound fluids, was significantly higher in group treated with 1% gall extract gel in comparison to the control group (=0.026).

CONCLUSION

Our results could not prove the significant positive effect of galls extract on the burning wound healing. More studies with more groups treated with different doses of the extract are recommended.

摘要

目的

本研究旨在评估五倍子提取物对大鼠皮肤烧伤创面愈合的影响。

材料与方法

采用五倍子乙醇提取物对32只Wistar大鼠背部诱导的烧伤创面进行治疗，将大鼠分为4组。分别用安慰剂、1%、2%和4%浓度的五倍子提取物凝胶治疗14天，并根据烧伤创面面积的减少以及组织学和分子特征评估治疗效果。

结果

在第14天，所有用五倍子提取物治疗的组的平均创面面积均大于对照组，差异具有统计学意义（P = 0.043）。平均组织学创面愈合评分无统计学差异。研究第5天时对创面渗出液中一氧化氮和血小板衍生生长因子浓度的分析表明，各组之间无显著差异（分别为P = 0.468和0.312）。与对照组相比，用1%五倍子提取物凝胶治疗的组创面渗出液中成纤维细胞生长因子（bFGF）浓度显著更高（P = 0.026）。