In Vitro Evaluation of Antioxidant Activity and Antibacterial Effects and Measurement of Total Phenolic and Flavonoid Contents of L. Fruit Extract.

Plant-based extracts, as alternatives to chemical compounds, are commonly use in pharmaceutical and food industries. Antibacterial properties of extracts are mainly considered in medicine. Because of the high incidence of infectious diseases, it is helpful to identify more agents that are able to treat diseases. Antioxidant effects have been reported for different plant extracts. We aimed to investigate antioxidant activity, total phenolic and flavonoid contents, and antibacterial effect of L. fruit extract. In this study, L. fruit was extracted by maceration using ethanol 70%. Total phenolic content was determined by Folin-Ciocalceu reagent and gallic acid equivalence, and antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) compared to butylated hydroxytoluene (BHT). Antibacterial effects were investigated by broth microdilution and measurement of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Then the effects of different concentrations of extract on and growth were investigated in comparison with vancomycin and nitrofurantoin. Our findings demonstrate that antioxidant activity of hydroalcoholic extract of L. fruit was more than that of BHT. Total phenolic content was derived (3.010 mg GAE/g DW). Flavonol and flavonoid contents were 1.813 and 0.654 mg/g, respectively. The extract was found to exert significant inhibitory effects on both studied bacteria. was more susceptible than to L. fruit extract. L. fruit extract can exert considerable antioxidant effects, contains high amounts of phenolic, flavonol, and flavonoid compounds, and inhibits bacterial growth.