Khanavi Mahnaz, Sabbagh-Bani-Azad Mansoureh, Abdolghaffari Amir Hossein, Vazirian Mahdi, Isazadeh Isa, Rezvanfar Mohammad Amin, Baeeri Maryam, Mohammadirad Azadeh, Rahimi Roja, Shams-Ardekani Mohammad Reza, Abdollahi Mohammad
Department of Pharmacognosy, Faculty of Pharmacy, and Medicinal Plants Research Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
Pharmacology and Applied Medicine, Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran ; International Campus, Tehran University of Medical Sciences, Tehran, Iran.
Arch Med Sci. 2014 Dec 22;10(6):1225-34. doi: 10.5114/aoms.2014.47831.
In this study we investigated the effect of gall of Quercus brantii Lindl., a traditional Iranian medicine, in a murine model of experimental colitis induced in male rats by rectal administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS).
Quantification of the main active components was done for estimation of total phenolic content and free gallic acid. Gall of Quercus brantii Lindl. in two forms (gall powder and gall hydro alcoholic extract) was gavaged for 10 days (500 mg/kg). Ten days after induction of colitis, colonic status was examined by macroscopic, microscopic and biochemical analyses. Colonic tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were analyzed as biomarkers of inflammatory condition. To determine the role of oxidative stress (OS) in colitis, the levels of cellular lipid peroxidation (LPO), total antioxidant power (TAP) and myeloperoxidase (MPO) were measured in colon tissues.
TNBS-induced colitis exhibited a significant increase in colon MPO activity and concentrations of cellular LPO, TNF-α and IL-1β, while TAP was significantly reduced. Microscopic evaluations of the colonic damage in the colitis group revealed multifocal degenerative changes in the epithelial lining and areas of necrosis, extensive mucosal and sub-mucosal damage with congested blood vessels, edema and hemorrhages along with extensive infiltration of inflammatory cells. Parameters including macroscopic and microscopic scores, TNF-α, IL-1β, LPO, TAP and MPO improved by both gall extract and gall powder of Quercus brantii Lindl. and reached close to normal levels. The level of total phenols (GAE/100 g of sample) and free gallic acid were estimated to be 88.43 ±7.23 (mean ± SD) and 3.74% of dry weight, respectively.
The present study indicates that the gall of Quercus brantii Lindl. is able to exert antioxidative and anti-inflammatory effects on the biochemical and pathological parameters of colitis.
在本研究中，我们研究了伊朗传统药物布兰特栎（Quercus brantii Lindl.）的没食子在雄性大鼠中通过直肠给予2,4,6-三硝基苯磺酸（TNBS）诱导的实验性结肠炎小鼠模型中的作用。
对主要活性成分进行定量，以估计总酚含量和游离没食子酸。以两种形式（没食子粉末和没食子水醇提取物）给予布兰特栎的没食子，持续10天（500毫克/千克）。在诱导结肠炎10天后，通过宏观、微观和生化分析检查结肠状况。分析结肠肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）作为炎症状态的生物标志物。为了确定氧化应激（OS）在结肠炎中的作用，测量结肠组织中细胞脂质过氧化（LPO）、总抗氧化能力（TAP）和髓过氧化物酶（MPO）的水平。
TNBS诱导的结肠炎表现为结肠MPO活性以及细胞LPO、TNF-α和IL-1β浓度显著增加，而TAP显著降低。对结肠炎组结肠损伤的微观评估显示上皮内衬多灶性退行性变化和坏死区域，广泛的黏膜和黏膜下损伤，伴有血管充血、水肿和出血，以及炎症细胞的广泛浸润。包括宏观和微观评分、TNF-α、IL-1β、LPO、TAP和MPO在内的参数通过布兰特栎的没食子提取物和没食子粉末均得到改善，并接近正常水平。总酚（GAE/100克样品）和游离没食子酸的水平估计分别为88.43±7.23（平均值±标准差）和干重的3.74%。
本研究表明，布兰特栎的没食子能够对结肠炎的生化和病理参数发挥抗氧化和抗炎作用。
