用于研究抗生素耐药性的功能宏基因组学
Functional Metagenomics to Study Antibiotic Resistance.
作者信息
Boolchandani Manish, Patel Sanket, Dantas Gautam
机构信息
Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
出版信息
Methods Mol Biol. 2017;1520:307-329. doi: 10.1007/978-1-4939-6634-9_19.
The construction and screening of metagenomic expression libraries has great potential to identify novel genes and their functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.
宏基因组表达文库的构建与筛选在鉴定新基因及其功能方面具有巨大潜力。在此，我们描述了从粪便DNA制备宏基因组文库、筛选文库中的抗生素抗性基因（ARG）、对富集的DNA片段进行大规模平行DNA测序，以及用于功能选择DNA的高通量组装和注释的计算流程。
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