功能宏基因组学在抗生素耐药性研究中的应用。
Functional Metagenomics to Study Antibiotic Resistance.
机构信息
The Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
出版信息
Methods Mol Biol. 2023;2601:379-401. doi: 10.1007/978-1-0716-2855-3_21.
The construction and screening of metagenomic expression libraries have a great potential to identify novel genes with desired functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.
宏基因组表达文库的构建和筛选具有很大的潜力，可以鉴定具有所需功能的新基因。在这里，我们描述了从粪便 DNA 中制备宏基因组文库、筛选文库中抗生素抗性基因（ARGs）、对富集的 DNA 片段进行大规模平行 DNA 测序以及用于高通量组装和功能选择 DNA 注释的计算流程。
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