Department of Ophthalmology, Institute for Clinical and Experimental Medicine, Faculty of Health Sciences, Linkoping University, Linköping 58183, Sweden.
National Bioinformatics Infrastructure Sweden (NBIS) Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm S-10691, Sweden.
Sci Data. 2016 Nov 22;3:160103. doi: 10.1038/sdata.2016.103.
In angiogenesis with concurrent inflammation, many pathways are activated, some linked to VEGF and others largely VEGF-independent. Pathways involving inflammatory mediators, chemokines, and micro-RNAs may play important roles in maintaining a pro-angiogenic environment or mediating angiogenic regression. Here, we describe a gene expression dataset to facilitate exploration of pro-angiogenic, pro-inflammatory, and remodelling/normalization-associated genes during both an active capillary sprouting phase, and in the restoration of an avascular phenotype. The dataset was generated by microarray analysis of the whole transcriptome in a rat model of suture-induced inflammatory corneal neovascularisation. Regions of active capillary sprout growth or regression in the cornea were harvested and total RNA extracted from four biological replicates per group. High quality RNA was obtained for gene expression analysis using microarrays. Fold change of selected genes was validated by qPCR, and protein expression was evaluated by immunohistochemistry. We provide a gene expression dataset that may be re-used to investigate corneal neovascularisation, and may also have implications in other contexts of inflammation-mediated angiogenesis.
在伴有炎症的血管生成中，许多途径被激活，其中一些与 VEGF 有关，而另一些则主要与 VEGF 无关。涉及炎症介质、趋化因子和 microRNAs 的途径可能在维持促血管生成环境或介导血管生成消退方面发挥重要作用。在这里，我们描述了一个基因表达数据集，以促进在活跃的毛细血管发芽阶段和恢复无血管表型期间探索促血管生成、促炎和重塑/正常化相关基因。该数据集是通过对大鼠缝线诱导的炎症性角膜新生血管化模型的全转录组进行微阵列分析生成的。从每组的四个生物学重复中收获活跃的毛细血管芽生长或消退的区域，并从每个区域提取总 RNA。使用微阵列进行基因表达分析获得了高质量的 RNA。通过 qPCR 验证了选定基因的倍数变化，并通过免疫组织化学评估了蛋白质表达。我们提供了一个基因表达数据集，可重新用于研究角膜新生血管化，并且在炎症介导的血管生成的其他情况下也可能具有意义。
