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miRNA 和 mRNA 在 Rag2 基因敲除和野生型小鼠脾脏中的表达的基因芯片分析

Microarray profiling of miRNA and mRNA expression in Rag2 knockout and wild-type mouse spleens.

机构信息

Department of Stem Cell and Regenerative Biotechnology, Humanized Pig Research Center (SRC), Konkuk University, Seoul 143-701, Republic of Korea.

Department of Animal Science, Busan National University, Miryang, Gyeongnam 50463, Republic of Korea.

出版信息

Sci Data. 2018 Jan 9;5:170199. doi: 10.1038/sdata.2017.199.

DOI:10.1038/sdata.2017.199
PMID:29313843
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5759371/
Abstract

The Rag2 knockout (KO) mouse is one of the most popular immune compromised animal models used in biomedical research. The immune compromised state concurrently alters many signalling pathways and molecules, including miRNAs and mRNA transcripts that are involved in important biological processes. In addition, miRNAs and transcripts are interdependent, often forming a feedback loop; dysregulation in one might alter the expression of the other, and both participate in many physiological processes including immune regulation. Here, we describe a comprehensive dataset containing alterations in the expression of both miRNAs and mRNAs in Rag2 KO mice compared to their wild type counterparts. The miRNA and mRNA expression profiles were generated from total RNA using a miRNA expression microarray or a BeadChip microarray, respectively. Hence, this dataset will provide the groundwork for a comparative study of the miRNAs and mRNAs that are dysregulated in Rag2 KO mice. It is hoped that the data will illuminate how miRNAs mediate immune regulation, as well as the interaction between miRNAs and mRNAs in Rag2 KO mice.

摘要

Rag2 敲除（KO）小鼠是生物医学研究中使用最广泛的免疫缺陷动物模型之一。免疫缺陷状态同时改变了许多信号通路和分子，包括 miRNA 和参与重要生物学过程的 mRNA 转录本。此外，miRNA 和转录本相互依存，经常形成反馈回路；一方的失调可能改变另一方的表达，两者都参与许多生理过程，包括免疫调节。在这里，我们描述了一个包含 Rag2 KO 小鼠与野生型对照相比，miRNA 和 mRNA 表达变化的综合数据集。miRNA 和 mRNA 的表达谱分别使用 miRNA 表达微阵列或 BeadChip 微阵列从总 RNA 中生成。因此，该数据集将为 Rag2 KO 小鼠中失调的 miRNA 和 mRNA 的比较研究提供基础。希望这些数据能够阐明 miRNA 如何介导免疫调节，以及 Rag2 KO 小鼠中 miRNA 和 mRNA 之间的相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/2e49188d89aa/sdata2017199-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/0a3b67c778eb/sdata2017199-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/987ce8b286f3/sdata2017199-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/8bbabb473891/sdata2017199-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/cf15f1d8a292/sdata2017199-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/c092992efb86/sdata2017199-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/2e49188d89aa/sdata2017199-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/0a3b67c778eb/sdata2017199-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/987ce8b286f3/sdata2017199-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/8bbabb473891/sdata2017199-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/cf15f1d8a292/sdata2017199-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/c092992efb86/sdata2017199-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac37/5759371/2e49188d89aa/sdata2017199-f6.jpg

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