Tazzari P L, Battelli M G, Abbondanza A, Dinota A, Rizzi S, Gobbi M, Stirpe F
Istituto di Ematologia Seràgnoli, Universitá di Bologna, Italy.
Transplantation. 1989 Jul;48(1):119-22. doi: 10.1097/00007890-198907000-00028.
We report on the preparation of an antibody-conjugated enzyme consisting of xanthine oxidase, a free-radical-producing enzyme, linked to the 62B1 monoclonal antibody, which recognizes the last steps of differentiation of B cell lineage (plasma cell and hairy cells). The conjugate specifically kills target cells, retaining both enzymic and immunological properties, without any damage to normal myeloid clonogenic efficiency. The model is suitable for ex vivo bone marrow purging in multiple myeloma patients.
我们报告了一种抗体偶联酶的制备方法，该酶由黄嘌呤氧化酶（一种产生自由基的酶）与62B1单克隆抗体连接而成，62B1单克隆抗体可识别B细胞谱系分化的最后阶段（浆细胞和毛细胞）。该偶联物能特异性杀死靶细胞，同时保留酶活性和免疫特性，且不会对正常髓系克隆形成效率造成任何损害。该模型适用于多发性骨髓瘤患者的体外骨髓净化。
