Honma Y, Kasukabe T, Hozumi M
Gan. 1977 Aug;68(4):405-12.
Glucorticoid hormones induced differentiation of mouse myeloid leukemia cells. From comparison of the structure of steroids with their ability to induce phagocytic and locomotive activities, typical characters of mature macrophages and granulocytes, the simplest steroid with inducing ability was concluded to be a steroid with the structure of progesterone and one hydroxyl group at 11beta- or 21-position. The maximum induction ability seemed to require the structure of progesterone and three hydroxyl groups (at 11beta-, 17alpha-, and 21-positions). A single, 30-min pulse treatment with glucocorticoid was sufficient to induce differentiation of leukemia cells. Glucocorticoid receptors were detected in mouse myeloid leukemia cells. The binding affinity of various steroids for the cytoplasmic receptors was closely correlated with the activities of these compounds to induce differentiation of leukemia cells, suggesting that these receptors may be involved in hormonal induction of differentiation of various cells. This suggests that the binding reaction is important for differentiation of myeloid leukemia cells.
糖皮质激素诱导小鼠髓系白血病细胞分化。通过比较类固醇的结构与其诱导吞噬和运动活性（成熟巨噬细胞和粒细胞的典型特征）的能力，得出具有诱导能力的最简单类固醇是具有孕酮结构且在11β-或21位有一个羟基的类固醇。最大诱导能力似乎需要孕酮结构和三个羟基（在11β-、17α-和21位）。用糖皮质激素进行单次30分钟的脉冲处理足以诱导白血病细胞分化。在小鼠髓系白血病细胞中检测到糖皮质激素受体。各种类固醇与细胞质受体的结合亲和力与这些化合物诱导白血病细胞分化的活性密切相关，这表明这些受体可能参与激素诱导的各种细胞分化。这表明结合反应对髓系白血病细胞的分化很重要。
