Abe E, Miyaura C, Sakagami H, Takeda M, Konno K, Yamazaki T, Yoshiki S, Suda T
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4990-4. doi: 10.1073/pnas.78.8.4990.
Mouse myeloid leukemia cells can be induced to differentiate into macrophages in vitro by 1 alpha,25-dihydroxyvitamin D3, the active form of vitamin D3. The minimal concentration of 1 alpha,25-dihydroxyvitamin D3 to induce the cell differentiation was 0.12 nM. The degree of cell differentiation in various markers induced by 12 nM 1 alpha,25-dihydroxyvitamin D3 was nearly equivalent to that induced by 1 microM dexamethasone, the most potent known stimulator. Among several markers of the differentiation by 1 alpha,25-dihydroxyvitamin D3, phagocytic activity was induced within 24 hr, and this was followed by induction of lysozyme and locomotive activities. Similar changes were also induced by 0.01-1 microM 1 alpha-hydroxyvitamin D3. 25-Hydroxyvitamin D3 and 24R,25-dihydroxyvitamin D3 showed only weak inducing activity. These results suggest the possibility that, in addition to its wellknown biological activities in enhancing intestinal calcium transport and bone mineral mobilization, 1 alpha, 25-dihydroxyvitamin D3 is involved in the differentiation of bone marrow cells.
小鼠髓性白血病细胞可在体外被维生素D3的活性形式1α,25 - 二羟基维生素D3诱导分化为巨噬细胞。诱导细胞分化的1α,25 - 二羟基维生素D3的最低浓度为0.12 nM。12 nM的1α,25 - 二羟基维生素D3诱导的各种标志物的细胞分化程度与已知最有效的刺激物1 μM地塞米松诱导的程度几乎相当。在1α,25 - 二羟基维生素D3诱导分化的几个标志物中，吞噬活性在24小时内被诱导，随后溶菌酶和运动活性被诱导。0.01 - 1 μM的1α - 羟基维生素D3也诱导了类似的变化。25 - 羟基维生素D3和24R,25 - 二羟基维生素D3仅表现出微弱的诱导活性。这些结果表明，除了其在增强肠道钙转运和骨矿物质动员方面众所周知的生物学活性外，1α,25 - 二羟基维生素D3还参与骨髓细胞的分化。
