Ball G F, Faris P L, Wingfield J C
Rockefeller University Field Research Center, Millbrook, New York.
Cell Tissue Res. 1989 Jul;257(1):155-61. doi: 10.1007/BF00221646.
Corticotropin-releasing factor (CRF) was localized in the brains of two passerine species, the European starling (Sturnus vulgaris) and the song sparrow (Melospiza melodia), by means of immunohistochemistry. The hypothalamic distribution of this peptide in these species includes a complex of immunoreactive perikarya observed in the paraventricular nucleus (PVN), in both its medial and lateral divisions. Nerve fibers were also seen running from these areas to the anterior median eminence (AME) where a terminal field is apparent. A wide variety of extrahypothalamic nuclei containing CRF-immunoreactive cells and fibers were identified. An apparent CRF terminal field can be visualized in the lateral septum. A dense fiber plexus is present in the nucleus accumbens (Ac) and more caudally in the nucleus of the stria terminalis (nST). In colchicine-pretreated animals, it was revealed that these areas also contain CRF-stained perikarya. The pattern of CRF immunoreactivity in the Ac-nST complex is continuous, with no distinction apparent between the nuclei. The medial preoptic area (mPOA) and the adjacent diagonal band of Broca contain CRF-fibers, while cells are apparent in the mPOA. In the mesencephalon, cells were visualized in the midbrain central gray; a terminal field and scattered positively stained perikarya were found in areas more ventral to the central grey that are adjacent to the third cranial nerve. Scattered cells were also seen at the border of the nucleus intercollicularis-nucleus mesencephalicus lateralis, pars dorsalis complex. In contrast to mammalian studies, no immunoreactive nerve fibers or perikarya were observed in telencephalic areas homologous to the mammalian neocortex.(ABSTRACT TRUNCATED AT 250 WORDS)
通过免疫组织化学方法，在两种雀形目鸟类——欧洲椋鸟（Sturnus vulgaris）和歌带鹀（Melospiza melodia）的大脑中定位了促肾上腺皮质激素释放因子（CRF）。在这些物种中，这种肽在下丘脑的分布包括在室旁核（PVN）的内侧和外侧分区观察到的一群免疫反应阳性的神经元胞体。还可见神经纤维从这些区域延伸至前正中隆起（AME），此处有明显的终末场。鉴定出了多种含有CRF免疫反应性细胞和纤维的下丘脑外核团。在外侧隔可见明显的CRF终末场。伏隔核（Ac）中存在密集的纤维丛，在终纹床核（nST）更靠尾侧的部位也有。在秋水仙碱预处理的动物中发现，这些区域也含有CRF染色的神经元胞体。Ac-nST复合体中的CRF免疫反应模式是连续的，各核之间无明显区别。内侧视前区（mPOA）和相邻的布罗卡斜带含有CRF纤维，而mPOA中有明显的细胞。在中脑，中脑中央灰质中有细胞可见；在与第三脑神经相邻的中央灰质腹侧区域发现了终末场和散在的阳性染色神经元胞体。在间丘核-中脑外侧核背侧部复合体边界也可见散在细胞。与哺乳动物研究不同，在与哺乳动物新皮质同源的端脑区域未观察到免疫反应性神经纤维或神经元胞体。（摘要截短于250字）
