1 Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada.
2 Department of Chemistry, Faculty of Arts and Science, University of Toronto, Toronto, ON, Canada.
SLAS Technol. 2017 Apr;22(2):113-121. doi: 10.1177/2211068216680207. Epub 2016 Nov 23.
Conventional approaches to bacterial identification and drug susceptibility testing typically rely on culture-based approaches that take 2 to 7 days to return results. The long turnaround times contribute to the spread of infectious disease, negative patient outcomes, and the misuse of antibiotics that can contribute to antibiotic resistance. To provide new solutions enabling faster bacterial analysis, a variety of approaches are under development that leverage single-cell analysis, microfluidic concentration and detection strategies, and ultrasensitive readout mechanisms. This review discusses recent advances in this area and the potential of new technologies to enable more effective management of infectious disease.
传统的细菌鉴定和药敏试验方法通常依赖于基于培养的方法，需要 2 到 7 天才能得出结果。较长的周转时间导致了传染病的传播、患者的不良后果以及抗生素的滥用，这可能导致抗生素耐药性的产生。为了提供新的解决方案以实现更快的细菌分析，目前正在开发各种利用单细胞分析、微流控浓缩和检测策略以及超高灵敏度读出机制的方法。本文讨论了该领域的最新进展以及新技术在更有效地管理传染病方面的潜力。
