Kim Jongwook, Kim Mi-Bo, Yun Jun Gon, Hwang Jae Kwan
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
J Microbiol Biotechnol. 2017 Feb 28;27(2):242-250. doi: 10.4014/jmb.1610.10050.
Anti-photoaging effects of standardized extract (SGE) and its major active compound kirenol were investigated using Hs68 human dermal fibroblasts and hairless mice, respectively. UVB-irradiated hairless mice that received oral SGE (600 mg/kg/day) showed reduced wrinkle formation and skinfold thickness compared with the UVB-irradiated control. Furthermore, SGE treatment increased the mRNA levels of collagen synthesis genes (COL1A1, COL3A1, COL4A1, and COL7A1) and activated antioxidant enzyme (catalase), while suppressing matrix metalloproteinase (MMP-2, -3, -9, and -13) expression. In Hs68 fibroblasts, kirenol also significantly suppressed MMP expression while increasing the expression of COL1A1, COL3A1, and COL7A1. Collectively, our data demonstrate that both SGE and kirenol attenuated UVB-induced photoaging in hairless mice and fibroblasts through inhibition of the mitogen-activated protein kinases and nuclear factor kappa B pathways, suggesting that SGE has potential to serve as a natural anti-photoaging nutraceutical.
分别使用Hs68人真皮成纤维细胞和无毛小鼠，研究了标准化提取物（SGE）及其主要活性化合物奇壬醇的抗光老化作用。与接受紫外线B（UVB）照射的对照组相比，口服SGE（600毫克/千克/天）的UVB照射无毛小鼠的皱纹形成和皮褶厚度减少。此外，SGE处理增加了胶原蛋白合成基因（COL1A1、COL3A1、COL4A1和COL7A1）的信使核糖核酸（mRNA）水平并激活了抗氧化酶（过氧化氢酶），同时抑制基质金属蛋白酶（MMP-2、-3、-9和-13）的表达。在Hs68成纤维细胞中，奇壬醇也显著抑制MMP的表达，同时增加COL1A1、COL3A1和COL7A1的表达。总体而言，我们的数据表明，SGE和奇壬醇均通过抑制丝裂原活化蛋白激酶和核因子κB途径减轻UVB诱导的无毛小鼠和成纤维细胞的光老化，这表明SGE有潜力作为一种天然的抗光老化营养保健品。
