Ge Beilei, Mukherjee Sampa, Hsu Chih-Hao, Davis Johnnie A, Tran Thu Thuy T, Yang Qianru, Abbott Jason W, Ayers Sherry L, Young Shenia R, Crarey Emily T, Womack Niketta A, Zhao Shaohua, McDermott Patrick F
Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA.
Food Microbiol. 2017 Apr;62:289-297. doi: 10.1016/j.fm.2016.10.029. Epub 2016 Oct 18.
Methicillin-resistant Staphylococcus aureus (MRSA) has been detected in retail meats, although large-scale studies are scarce. We conducted a one-year survey in 2010-2011 within the framework of the National Antimicrobial Resistance Monitoring System. Among 3520 retail meats collected from eight U.S. states, 982 (27.9%) contained S. aureus and 66 (1.9%) were positive for MRSA. Approximately 10.4% (107/1032) of S. aureus isolates, including 37.2% (29/78) of MRSA, were multidrug-resistant (MDRSA). Turkey had the highest MRSA prevalence (3.5%), followed by pork (1.9%), beef (1.7%), and chicken (0.3%). Whole-genome sequencing was performed for all 66 non-redundant MRSA. Among five multilocus sequence types identified, ST8 (72.7%) and ST5 (22.7%) were most common and livestock-associated MRSA ST398 was assigned to one pork isolate. Eleven spa types were represented, predominately t008 (43.9%) and t2031 (22.7%). All four types of meats harbored t008, whereas t2031 was recovered from turkey only. The majority of MRSA (84.8%) possessed SCCmec IV and 62.1% harbored Panton-Valentine leukocidin. Pulsed-field gel electrophoresis showed that all ST8 MRSA belonged to the predominant human epidemic clone USA300, and others included USA100 and USA200. We conclude that a diverse MRSA population was present in U.S. retail meats, albeit at low prevalence.
零售肉类中已检测到耐甲氧西林金黄色葡萄球菌（MRSA），不过大规模研究较为匮乏。我们在2010 - 2011年于国家抗菌药物耐药性监测系统框架内开展了为期一年的调查。在从美国八个州采集的3520份零售肉类中，982份（27.9%）含有金黄色葡萄球菌，66份（1.9%）MRSA呈阳性。约10.4%（107/1032）的金黄色葡萄球菌分离株为多重耐药（MDRSA），其中包括37.2%（29/78）的MRSA。火鸡的MRSA患病率最高（3.5%），其次是猪肉（1.9%）、牛肉（1.7%）和鸡肉（0.3%）。对所有66株非冗余MRSA进行了全基因组测序。在鉴定出的五种多位点序列类型中，ST8（72.7%）和ST5（22.7%）最为常见，与牲畜相关的MRSA ST398被鉴定为一株猪肉分离株。共出现了11种spa型，主要是t008（43.9%）和t2031（22.7%）。所有四种肉类均含有t008，而t2031仅从火鸡中分离得到。大多数MRSA（84.8%）携带SCCmec IV，62.1%含有杀白细胞素。脉冲场凝胶电泳显示，所有ST8 MRSA均属于主要的人类流行克隆USA300，其他还包括USA100和USA200。我们得出结论，美国零售肉类中存在多种MRSA菌群，尽管患病率较低。
