Rueda Alexandra, Realpe Emilio, Uribe Alfredo
Biological Sciences Department, Laboratory of Zoology and Aquatic Ecology LAZOEA, Universidad de los Andes, Bogotá, Colombia.
Biological Sciences Department, Biochemical Investigation Center CIBI, Universidad de los Andes, Bogotá, Colombia.
Toxicon. 2017 Jan;125:53-58. doi: 10.1016/j.toxicon.2016.11.255. Epub 2016 Nov 23.
The genus Latrodectus has not been studied in Colombia even though it is medically important worldwide; there are three species for the country, this study focused on a non-identified species found in the Tatacoa Desert in the Huila Department. This research is the first approximation to the extraction, composition analysis and toxicity evaluation of the venom of a species of the genus Latrodectus in Colombia; and aims to evaluate the toxicity by the initial characterization of its venom. The venom extraction was accomplished with electrostimulation and total protein concentration was determined by the Lowry method and BCA assays from crude venom; with these methods, high protein concentration of the samples was measured. Bioassays on mice were also made to evaluate the toxicity and compare the symptoms produced by this Colombian spider to the Latrodectism Syndrome. Finally, an SDS-PAGE electrophoresis was used to separate the main components of high molecular weight from the samples and compared to a control of the venom of Latrodectus mactans to determine if the venom composition is different between these two species.
尽管寇蛛属在全球具有医学重要性，但在哥伦比亚尚未对其进行研究；该国存在三个物种，本研究聚焦于在乌伊拉省塔塔科阿沙漠发现的一个未鉴定物种。这项研究是对哥伦比亚寇蛛属一种蜘蛛毒液的提取、成分分析和毒性评估的首次探索；旨在通过对其毒液的初步表征来评估毒性。毒液提取通过电刺激完成，粗毒液中的总蛋白浓度通过洛瑞法和BCA测定法确定；通过这些方法，测量了样品中的高蛋白浓度。还对小鼠进行了生物测定以评估毒性，并将这种哥伦比亚蜘蛛产生的症状与Latrodectism综合征进行比较。最后，使用SDS - PAGE电泳从样品中分离高分子量的主要成分，并与黑寡妇蜘蛛毒液对照进行比较，以确定这两个物种之间的毒液成分是否不同。
