Duan Zhigui, Yan Xiaojun, Cao Rui, Liu Zhen, Wang Xianchun, Liang Songping
College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, People's Republic of China.
J Biochem Mol Toxicol. 2008 Sep-Oct;22(5):328-36. doi: 10.1002/jbt.20244.
Black widow spider is one of the most poisonous spiders in the world. Up to now, there have been few systematic analyses of the spider venom components, and the mechanism of action of the venom has not been completely understood. In this work, we employed combinative proteomic strategy to analyze the venom collected from living adult spider Latrodectus tredecimguttatus by electrical stimulation. The experiments demonstrated that the venom is primarily composed of high molecular weight proteins and has high abundance proteins around 100 kDa. The content of peptides and proteins with low molecular weight is low. A total of 75 nonredundant venom proteins with distinct function were unambiguously identified. Besides the known black widow spider venom proteins including latrotoxins, a variety of hydrolases and other proteins with special activity were found in the venom, such as proteinase, phospholipase, phosphatase, nuclease, fucolectin, venom allergen antigen 5-like protein and trypsin inhibitor, and so on. Their possible biological actions and relationship with latrodectism were discussed. The results help to understand the complexity and action mechanism of L. tredecimguttatus venom.
黑寡妇蜘蛛是世界上毒性最强的蜘蛛之一。到目前为止，对蜘蛛毒液成分的系统分析很少，毒液的作用机制也尚未完全了解。在这项工作中，我们采用组合蛋白质组学策略，通过电刺激分析从成年活体红斑寇蛛采集的毒液。实验表明，毒液主要由高分子量蛋白质组成，并且在100 kDa左右有高丰度蛋白质。低分子量的肽和蛋白质含量较低。共明确鉴定出75种具有不同功能的非冗余毒液蛋白。除了已知的黑寡妇蜘蛛毒液蛋白，包括α- latrotoxins，在毒液中还发现了多种水解酶和其他具有特殊活性的蛋白质，如蛋白酶、磷脂酶、磷酸酶、核酸酶、岩藻凝集素、毒液变应原抗原5样蛋白和胰蛋白酶抑制剂等。讨论了它们可能的生物学作用以及与黑寡妇蜘蛛中毒的关系。这些结果有助于了解红斑寇蛛毒液的复杂性和作用机制。
