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使用特异性16S rRNA基因PCR引物快速鉴定皮奥里亚罗宾逊菌。

Rapid identification of Robinsoniella peoriensis using specific 16S rRNA gene PCR primers.

作者信息

Whitehead Terence R, Anoma Christelle, McLaughlin Richard W

机构信息

Bioenergy Research Unit, National Center for Agricultural Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL, 61604, USA.

General Studies, Gateway Technical College, Kenosha, WI, 53144, USA.

出版信息

Anaerobe. 2017 Feb;43:39-42. doi: 10.1016/j.anaerobe.2016.11.008. Epub 2016 Nov 24.

Abstract

Robinsoniella peoriensis is a Gram-positive, spore-forming anaerobic bacterium initially isolated and characterized from swine manure and feces. Since then strains of this species have been identified from a variety of mammalian and other GI tracts. More recently strains of this species have been isolated from a plethora of human infections. Therefore, it is of great interest to develop methods to rapidly identify this microorganism in the medical and other laboratories. This report describes the use of PCR primers targeting the 16S rRNA gene of R. peoriensis to identify strains of this bacterium.

摘要

皮奥里亚罗宾逊氏菌是一种革兰氏阳性、形成芽孢的厌氧细菌,最初从猪粪和粪便中分离并鉴定出来。从那时起,已从多种哺乳动物和其他胃肠道中鉴定出该物种的菌株。最近,该物种的菌株已从大量人类感染中分离出来。因此,开发在医学和其他实验室中快速鉴定这种微生物的方法具有极大的意义。本报告描述了使用针对皮奥里亚罗宾逊氏菌16S rRNA基因的PCR引物来鉴定该细菌的菌株。

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