转化生长因子-β对小鼠系膜细胞增殖的影响。
The effect of transforming growth factor-beta on mouse mesangial cell proliferation.
作者信息
Yamashita W, MacCarthy E P, Hsu A, Gartside P S, Ooi B S
机构信息
Department of Internal Medicine, University of Cincinnati Medical Center, Ohio 45267-0585.
出版信息
Clin Exp Immunol. 1989 Aug;77(2):285-8.
The factors which regulate mesangial cell growth are of importance in determining the cellular lesions of nephritis. In this communication, we report that transforming growth factor-beta (TGF-beta) exerts a suppressive effect on mesangial cell 3H-thymidine uptake. Furthermore, TGF-beta antagonizes the mitogenic effect of epidermal growth factor (EGF) on DNA synthesis. The concomitant presence of TGF-beta in the cell cultures is not required for its effect since cells pretreated with the substance and rinsed of it showed suppressed 3H-thymidine uptake and did not respond to EGF. The effect of TGF-beta can also be demonstrated on cells committed to proliferate by the prior addition of EGF. The results of the study have relevance to the mechanisms underlying the histologic lesions of immune-mediated nephritis.
调节系膜细胞生长的因素对于确定肾炎的细胞病变至关重要。在本报告中，我们指出转化生长因子-β（TGF-β）对系膜细胞的3H-胸腺嘧啶核苷摄取具有抑制作用。此外，TGF-β可拮抗表皮生长因子（EGF）对DNA合成的促有丝分裂作用。细胞培养物中同时存在TGF-β并非其发挥作用所必需，因为用该物质预处理并冲洗后的细胞显示3H-胸腺嘧啶核苷摄取受到抑制，且对EGF无反应。TGF-β的作用也可在预先添加EGF而致力于增殖的细胞上得到证实。该研究结果与免疫介导性肾炎组织学病变的潜在机制相关。
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