MacCarthy E P, Hsu A, Ooi Y M, Ooi B S
Immunology. 1985 Dec;56(4):695-9.
Mesangial hypercellularity is usually found in many models of nephritis characterized by monocyte/macrophage infiltration of the glomerulus. In order to examine the mechanism mediating these events, an in vitro model was used to study the effects of macrophage products on mouse mesangial cells, cultured under conditions which would render them relatively quiescent. Under these conditions, macrophage supernatants stimulated the proliferation of the mesangial cells. The stimulatory effect could be shown to be due in part to enhancement of endogenous mesangial cell PGE production. This was demonstrated by experiments which showed that macrophage supernatants stimulated mesangial cell PGE production, that the stimulatory effect of macrophage products was abrogated by pretreatment of mesangial cells with indomethacin, and finally that exogenous PGE2 stimulated mesangial cell proliferation.
系膜细胞增多通常见于许多以肾小球单核细胞/巨噬细胞浸润为特征的肾炎模型中。为了研究介导这些事件的机制，我们使用了一种体外模型来研究巨噬细胞产物对小鼠系膜细胞的影响，这些系膜细胞在相对静止的条件下培养。在这些条件下，巨噬细胞上清液刺激了系膜细胞的增殖。刺激作用部分归因于内源性系膜细胞前列腺素E（PGE）生成的增强。实验表明，巨噬细胞上清液刺激系膜细胞PGE生成，用吲哚美辛预处理系膜细胞可消除巨噬细胞产物的刺激作用，最后外源性PGE2刺激系膜细胞增殖，这些实验证实了这一点。
