García G, Dogi C, de Moreno de LeBlanc A, Greco C, Cavaglieri L
1 Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 km. 601, 5800 Río Cuarto, Córdoba, Argentina.
2 Fellow of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Benef Microbes. 2016 Nov 30;7(5):659-668. doi: 10.3920/BM2015.0071.
The aim was to evaluate the effect of Saccharomyces cerevisiae RC016 on immune parameters and gut microbiota in healthy mice. Animals received S. cerevisiae RC016 for 10 days. Microbial translocation to liver and changes in some bacterial populations in caecum were determined. Immune stimulation was assessed at gut level (measure ofimmunoglobulin A (IgA) cells and luminal cytokine profile) and by evaluating the activity of peritoneal macrophages. Oral administration of S. cerevisiae RC016 did not induce microbial translocation to liver. Mice that received yeast increased the number of IgA cells in their intestines, the phagocytic activity of peritoneal macrophages and decreased tumour necrosis factor alpha (TNF-α) levels in the small intestine with increases of interleukin-10/TNF-αratio. Administration of S. cerevisiae RC016 caused the decline of a logarithmic unit for Enterobacteriaceae counts compared to the control. The immune and gut microbiota modulation observed demonstrates that S. cerevisiae RC016 is a promising candidate for the formulation of feed additives to improve animal productivity. The beneficial in vivo effects observed for the potential probiotic S. cerevisiae RC016 with previously reported mycotoxin-bindingproperties, demonstrated that this strain could be suitable to be included in a novel product to improve animalproductivity, with both probiotic and mycotoxin-binding properties. However, studies in the specific host will be necessary to confirm this potential.
目的是评估酿酒酵母RC016对健康小鼠免疫参数和肠道微生物群的影响。动物接受酿酒酵母RC016处理10天。测定微生物向肝脏的易位以及盲肠中一些细菌种群的变化。在肠道水平（免疫球蛋白A（IgA）细胞测量和肠腔细胞因子谱）评估免疫刺激，并通过评估腹腔巨噬细胞的活性来评估。口服酿酒酵母RC016未诱导微生物向肝脏易位。接受酵母的小鼠肠道中IgA细胞数量增加，腹腔巨噬细胞的吞噬活性增强，小肠中肿瘤坏死因子α（TNF-α）水平降低，白细胞介素-10/TNF-α比值升高。与对照组相比，给予酿酒酵母RC016导致肠杆菌科细菌计数下降一个对数单位。观察到的免疫和肠道微生物群调节表明，酿酒酵母RC016是一种有前途的饲料添加剂配方候选物，可提高动物生产力。对具有先前报道的霉菌毒素结合特性的潜在益生菌酿酒酵母RC016观察到的有益体内效应表明，该菌株可能适合纳入一种新型产品中，以提高动物生产力，兼具益生菌和霉菌毒素结合特性。然而，需要在特定宿主中进行研究以证实这种潜力。
