Richens E R, Shaltout A, Bahr G M, Abdella N, Jayyab A K, Al-Saffar M, Behbehani K
Department of Microbiology, Faculty of Medicine, University of Kuwait.
Acta Diabetol Lat. 1989 Apr-Jun;26(2):115-22. doi: 10.1007/BF02581363.
Insulin autoantibodies (IAAs) are associated with type I diabetes mellitus (DM) and have been suggested as predictive markers of the disease. Using an ELISA assay, we have studied the prevalence of binding to human insulin in sera from an Arab type I DM population and compared it with the prevalence in the family members (FMs) of the probands, in type II DM patients from the same population, and in Arab control subjects. Significant levels of binding occurred in 11/16 (69%) of type I DM patients and in 21/34 (62%) of their FMs, but in only 5/31 (16%) of type II DM patients and in 1/25 (4%) of control subjects. Within families, there was homogeneity with regard to the level of insulin binding and the mean family levels correlated with those of the proband (r = 0.68, df = 7, p = 0.05). HLA-DR3 or -DR4 antigens occurred in 55/63 (87%) of type I DM patients and in 95/118 (81%) of their FMs. This was significantly higher (p less than 0.001) than in either type II DM patients (39/75, 52%) or in control subjects (34/93, 37%). ICAs were present in significantly more (25/43, 58%) of type I DM patients than their FMs (3/82, 3%) (p less than 0.001). They did not occur in either type II DM patients or in the control group. In conclusion, insulin binding occurred in sera from both type I diabetic patients and their kindred, and hence did not appear to be specifically associated with the development of clinical diabetes.
胰岛素自身抗体（IAA）与1型糖尿病（DM）相关，并且已被认为是该疾病的预测标志物。我们使用酶联免疫吸附测定法（ELISA），研究了阿拉伯1型糖尿病患者血清中与人胰岛素结合的流行情况，并将其与先证者的家庭成员（FM）、同一人群中的2型糖尿病患者以及阿拉伯对照受试者中的流行情况进行了比较。16例1型糖尿病患者中有11例（69%）出现显著水平的结合，其34名FM中有21例（62%）出现显著水平的结合，但2型糖尿病患者中只有5例（16%）出现显著水平的结合，对照受试者中只有1例（4%）出现显著水平的结合。在家族内部，胰岛素结合水平具有同质性，且家族平均水平与先证者的水平相关（r = 0.68，自由度 = 7，p = 0.05）。HLA - DR3或 - DR4抗原出现在63例1型糖尿病患者中的55例（87%）及其118名FM中的95例（81%）。这显著高于2型糖尿病患者（75例中的39例，52%）或对照受试者（93例中的34例，37%）（p < 0.001）。1型糖尿病患者中ICA的出现比例显著高于其FM（43例中有25例，58%对比82例中有3例，3%）（p < 0.001）。它们在2型糖尿病患者或对照组中均未出现。总之，胰岛素结合出现在1型糖尿病患者及其亲属的血清中，因此似乎与临床糖尿病的发生没有特异性关联。
